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由于干旱和盐碱化的严重影响,我国大豆生产受到很大限制。为了提高大豆的抗旱性,培育抗旱转基因大豆新品种,利用农杆菌介导的子叶节遗传转化技术体系,首次将水稻热激蛋白基因HSP90导入大豆受体材料Bert中,通过HSP90在大豆中过表达,获得了耐旱转基因大豆新材料。本试验中4 000个子叶节外植体用于遗传转化,再生转化苗经PCR和Southern杂交鉴定结合PPT抗性筛选(bar为筛选标记),共获得128棵阳性转基因植株,转化率为3.2%。经初步筛选,获得15份耐旱性较好的材料,其耐旱性显著优于对照。研究结果为进一步筛选耐旱转基因大豆新材料奠定了较好的基础。
Due to the serious impact of drought and salinization, soybean production in our country is greatly restricted. In order to improve the drought resistance of soybean and cultivate a new drought-resistant transgenic soybean, the heat shock protein gene HSP90 was first introduced into soybean receptor material Bert by using agrobacterium-mediated cotyledonary node genetic transformation system and overexpressed in soybean through HSP90 , Obtained drought-tolerant genetically modified soybean new material. In this experiment, 4 000 explants of cotyledonary node were used for genetic transformation. Regenerated transformed plants were identified by PCR and Southern hybridization combined with PPT resistance screening (bar is a screening marker), a total of 128 positive transgenic plants were obtained, the transformation rate was 3.2% . After preliminary screening, 15 materials with better drought tolerance were obtained, whose drought tolerance was significantly better than that of the control. The results laid a good foundation for further screening of drought-tolerant transgenic soybean new material.