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目的探讨槲皮素与5-氨基水杨酸对感染后肠易激综合征大鼠的协同治疗价值。方法按治疗方案不同将50只大鼠分为5组,每组10只,即正常对照组、模型对照组、5-氨基水杨酸组、槲皮素组和槲皮素+5-氨基水杨酸组,连续给药2周,比较5组大鼠的内脏痛阈值和粪便含水率,血清及结肠5-氨基水杨酸和N-乙酰-5-氨基水杨酸含量,血清二胺氧化酶(DAO)及分泌型免疫球蛋白(SIgA)水平的变化情况。结果与正常对照组相比,模型对照组内脏痛阈值(30.64±3.98)mmHg明显降低,粪便含水率(59.42±7.28)%明显提高,血清DAO水平(16.34±0.81)U/mL明显提高,SIgA水平(15.22±0.82)mg/L明显降低,差异有统计学意义(P<0.05);与模型对照组相比,5-氨基水杨酸组、槲皮素组、槲皮素+5-氨基水杨酸组内脏痛阈值(39.05±4.75)mmHg、(39.37±4.82)mmHg、(41.81±5.03)mmHg明显提高,粪便含水率(50.11±6.79)%、(49.60±6.51)%、(47.43±6.34)%明显降低,血清DAO水平(15.19±0.78)U/mL、(15.15±0.76)U/mL、(14.87±0.75)U/mL明显降低,SIgA水平(16.40±0.87)mg/L、(16.56±0.81)mg/L、(17.10±0.90)mg/L明显提高,差异有统计学意义(P<0.05);与5-氨基水杨酸组相比,槲皮素+5-氨基水杨酸组结肠5-氨基水杨酸含量(2037.62±375.40)ng/g明显增加,N-乙酰-5-氨基水杨酸含量(1898.73±350.56)ng/g明显降低,差异有统计学意义(P<0.05)。结论槲皮素与5-氨基水杨酸对于感染后肠易激综合征大鼠症状体征改善及治疗效果的提升均具有极其重要的协同治疗价值。
Objective To investigate the synergistic therapeutic effect of quercetin and 5-aminosalicylic acid on irritable bowel syndrome (IBS) rats after infection. Methods Fifty rats were divided into five groups according to different treatment regimen, 10 in each group, namely, normal control group, model control group, 5-aminosalicylic acid group, quercetin group and quercetin + 5-amino water Salicylic acid group, continuous administration for 2 weeks, the visceral pain threshold and stool water content, serum and colon 5-aminosalicylic acid and N-acetyl-5-aminosalicylic acid content, serum diamine oxidation (DAO) and secretory immunoglobulin (SIgA) levels of the changes. Results Compared with the normal control group, the threshold of visceral pain (30.64 ± 3.98) mmHg in model control group was significantly decreased, the stool moisture content (59.42 ± 7.28)% was significantly increased, the serum DAO level (16.34 ± 0.81) U / mL was significantly increased, SIgA (15.22 ± 0.82) mg / L, the difference was statistically significant (P <0.05). Compared with the model control group, 5-aminosalicylic acid group, quercetin group, quercetin + 5- Salicylic acid group had significantly higher visceral pain threshold (39.05 ± 4.75) mmHg, (39.37 ± 4.82) mmHg and (41.81 ± 5.03) mmHg, and the stool water content was 50.11 ± 6.79%, 49.60 ± 6.51% and 47.43 ± 6.34)%, the level of serum DAO was significantly lower (15.19 ± 0.78) U / mL, (15.15 ± 0.76) U / mL, (14.87 ± 0.75) U / mL and the level of SIgA was (16.40 ± 0.87) mg / 16.56 ± 0.81) mg / L, (17.10 ± 0.90) mg / L, the difference was statistically significant (P <0.05). Compared with 5-aminosalicylic acid group, The concentration of 5-aminosalicylic acid in the acid group (2037.62 ± 375.40) ng / g was significantly increased, and the content of N-acetyl-5-aminosalicylic acid (1898.73 ± 350.56) ng / g was significantly decreased <0.05). Conclusions Both quercetin and 5-aminosalicylic acid have an extremely important synergistic therapeutic effect on the improvement of symptoms and signs and the therapeutic effect of irritable bowel syndrome after infection.