论文部分内容阅读
研究发现,莲藕根状茎干物质、可溶性糖及可溶性蛋白含量随着根状茎发育而逐步增加,尤其在根状茎发育后期含量增加更加明显。在此基础之上,利用转录组测序技术,归纳并分析86个可能与莲藕根状茎膨大相关基因,分别为35个激素诱导蛋白基因、4个光诱导蛋白(MADS-BOX)基因、11个根状茎贮藏蛋白基因(Patatin)、35个与淀粉代谢相关基因以及1个与根状茎形成相关基因。数字基因差异表达谱研究结果表明,根状茎贮藏蛋白家族基因(Lrplp6、Lrplp9、Lrpfp1、Lrprp、Lrpfp2、Lrplp4、Lrplp5、Lrplp8、Lrpp、Lrplp2)和淀粉合成相关基因(Lrgbss、Lrsbe1、Lrsbe2、LrsbeII、LrsbeIII)在根状茎形成后期表达量高,表达丰度均为初期的3倍以上,而其他基因变化相对较小。半定量RT-PCR结果进一步证明上述数字基因差异表达谱结果的可靠性,同时也表明Lrplp8和Lrgbss的表达与根状茎膨大具有高度相关性。由此认为,上述10个贮藏蛋白合成相关基因和5个淀粉合成相关基因,尤其Lrplp8和Lrgbss,对莲藕根状茎的膨大起到重要作用。
The results showed that the content of dry matter, soluble sugar and soluble protein of lotus root rhizome increased gradually with the development of rhizome, especially in late rhizome development. Based on this, 86 genes associated with rhizome bulking of the lotus root were summarized and analyzed using transcriptome sequencing technology, including 35 hormone-inducible protein genes, 4 light-induced proteins (MADS-BOX) genes and 11 Rhizome storage protein gene (Patatin), 35 genes related to starch metabolism and 1 gene related to rhizome formation. The results of the differential gene expression profiles showed that the genes of the rhizome storage protein family of genes (Lrplp6, Lrplp9, Lrpfp1, Lrprp, Lrpfp2, Lrplp4, Lrplp5, Lrplp8, Lrpp, Lrplp2) and starch synthesis related genes (Lrgbss, Lrsbe1, Lrsbe2, LrsbeII , LrsbeIII) were highly expressed at the late rhizome formation stage, and their expression abundance was more than 3 times that of the initial stage, while the changes of other genes were relatively small. The results of semi-quantitative RT-PCR further proved the reliability of the differential gene expression profiles of the above-mentioned digital genes and also showed that the expression of Lrplp8 and Lrgbss was highly correlated with the enlargement of rhizomes. Therefore, the above 10 storage protein synthesis related genes and 5 starch synthesis related genes, especially Lrplp8 and Lrgbss, played an important role in the swelling of lotus root rhizome.