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作者用酶联免疫吸附试验(ELISA),环卵沉淀试验(COPF)和对流免疫电泳(CIE)等方法观察了经胃蛋白酶-盐酸溶液处理的血吸虫卵在免疫诊断上的应用。从人尿中收集的埃及血吸虫卵经胃蛋白酶-盐酸溶液处理(pH1.8,37℃,2小时),除去虫卵表面的免疫沉淀物,然后用盐水洗涤。用于试验的有109例肯尼亚埃及血吸虫病人血清,105例曼氏血吸虫流行区居民血清及46例无血吸虫病病史的日本人血清,另有26例来自日本的肝吸虫、肺吸虫等其他寄生虫病病人血清用于交叉反应。结果,用ELISA,COPT和CIE检测埃及血吸虫病人血清的敏感度分别为92.3%,94.2%和
The authors used enzyme-linked immunosorbent assay (ELISA), egg enucleation test (COPF) and convection-immune electrophoresis (CIE) and other methods observed pepsin - hydrochloric acid solution of schistosome eggs in immunodiagnosis. Schistosoma japonicum eggs collected from human urine were treated with pepsin-hydrochloric acid solution (pH 1.8, 37 ° C, 2 hours) to remove immunoprecipitates from the surface of the eggs and then washed with saline. Serum samples from 109 Kengoan patients with schistosomiasis japonica, 105 from Schistosoma mansoni endemic sera and 46 from Japan without histories of schistosomiasis were also included in the test, and 26 other parasites such as liver flukes and paragonimiasis from Japan Patient serum is used for cross-reaction. As a result, the sera of patients with schistosomiasis japonica by ELISA, COPT and CIE were 92.3%, 94.2% and 94.2%, respectively