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目的探讨细胞凋亡在移植肝缺血再灌注损伤中的作用及缺血预处理对其影响。方法通过对移植肝进行缺血预处理，用全自动生化分析仪检测肝功能、比色法测定移植肝组织的ＭＤＡ、用流式细胞仪结合原位末端标记技术检测细胞凋亡。结果移植肝再灌注后血中ＡＳＴ，ＡＬＴ，ＬＤＨ和肝组织中ＭＤＡ均明显升高，肝细胞凋亡明显增加，经缺血预处理后，血中ＡＳＴ，ＡＬＴ，ＬＤＨ和肝组织中ＭＤＡ均降低，肝细胞凋亡亦明显减少。结论缺血预处理可以通过减轻移植肝脂质过氧化反应，从而减少肝细胞凋亡，使肝细胞损伤减轻从而保护移植肝。
Objective To investigate the role of apoptosis in liver ischemia-reperfusion injury and the effect of ischemic preconditioning on it. Methods The hepatic function was detected by automatic biochemical analyzer after ischemic preconditioning on the transplanted liver. The MDA level in the transplanted liver tissue was measured by colorimetric assay. The apoptosis was detected by flow cytometry and in situ end labeling. Results The levels of AST, ALT, LDH in blood and MDA in liver tissue were significantly increased after transplanted hepatic artery reperfusion and the apoptosis of hepatic cells was significantly increased. The contents of AST, ALT, LDH and MDA Reduce, hepatocyte apoptosis also significantly reduced. Conclusion Ischemic preconditioning can reduce the hepatocyte apoptosis by reducing the lipid peroxidation in the transplanted liver, so as to reduce the damage of hepatocytes and protect the transplanted liver.