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目的探讨血清瘦素、IL-13、嗜酸粒细胞趋化因子(Eotaxin)及总IgE(TIgE)在支气管哮喘(哮喘)发病中的作用及其关系。方法选择30例哮喘儿童(哮喘组)及22例健康儿童(健康对照组)为研究对象。二组年龄、性别、BMI比较差异均无统计学意义。ELISA法检测各组儿童血清IL-13、Eotaxin,放射免疫法检测各组儿童血清瘦素,荧光酶联免疫法检测其血清TIgE水平。哮喘患儿同时行肺功能检测。结果1.哮喘患儿急性期血清瘦素水平与健康对照组比较差异有统计学意义(P<0.05);哮喘患儿急性期血清瘦素水平与体质量指数(BMI)无明显相关性(r=0.330,P=0.075),哮喘患儿缓解期血清瘦素水平与BMI有明显相关性(r=0.565,P=0.001),健康对照组血清瘦素水平与BMI有明显相关性(r=0.732,P=0.000)。2.哮喘急性期与缓解期、哮喘缓解期与健康对照组之间血清Eotaxin、IL-13、TIgE水平差异均有统计学意义(Pa<0.001)。3.在哮喘急性期、缓解期血清Eotaxin与IL-13、Eotaxin与TIgE及IL-13与TIgE之间均呈正相关;血清瘦素与Eotaxin、IL-13及TIgE之间均无相关性。4.哮喘急性期、缓解期血清Eotaxin与一秒钟用力呼气量(FEV1)及呼气高峰流量(PEF)、血清IL-13与FEV1及PEF之间均呈负相关,血清瘦素与FEV1及PEF、血清TIgE与FEV1及PEF之间均无明显相关性。结论血清TIgE、IL-13及Eotaxin在哮喘发病中彼此相互影响,共同参与哮喘的病理生理改变,血清IL-13、Eotaxin可作为评判哮喘病情严重程度的一项客观指标。瘦素可能参与哮喘的发病。
Objective To investigate the role and relationship of serum leptin, IL-13, Eotaxin and total IgE (TIgE) in the pathogenesis of bronchial asthma (asthma). Methods Thirty asthmatic children (asthma group) and 22 healthy children (healthy control group) were selected as the research object. There was no significant difference between the two groups in age, sex and BMI. Serum levels of IL-13 and Eotaxin were detected by ELISA. Serum leptin level was detected by radioimmunoassay. Serum levels of TIgE were detected by fluorescence enzyme-linked immunosorbent assay. Children with asthma also perform pulmonary function tests. Serum leptin level in acute asthmatic children was significantly higher than that in healthy controls (P <0.05). There was no significant correlation between serum leptin levels and body mass index (BMI) in children with asthma (r = 0.330, P = 0.075). There was a significant correlation between serum leptin level and BMI in children with asthma (r = 0.565, P = 0.001), serum leptin level in healthy control group was significantly correlated with BMI , P = 0.000). Serum levels of Eotaxin, IL-13 and TIgE were significantly different between acute and remission stages of asthma and remission of asthma (P <0.001). There was a positive correlation between Eotaxin and IL-13, Eotaxin and TIgE, IL-13 and TIgE in acute asthmatic and remission stages; no correlation was found between serum leptin and Eotaxin, IL-13 and TIgE. Serum Eotaxin and FEV1, PEF, IL-13 and FEV1 and PEF in acute phase and acute phase of asthma were negatively correlated with serum leptin and FEV1 And PEF, serum TIgE and FEV1 and PEF no significant correlation between. Conclusion Serum levels of TIgE, IL-13 and Eotaxin may affect each other in the pathogenesis of asthma and participate in the pathophysiological changes of asthma. Serum IL-13 and Eotaxin may be used as objective indicators to evaluate the severity of asthma. Leptin may be involved in the pathogenesis of asthma.