目的:用体视学方法定量揭示肺癌细胞核的三维形态结构特点及变化规律。 方法 收集肺癌手术切除标本腺癌30例、鳞癌20例、腺鳞癌8例、未分化小细胞癌6例、未分化大细胞癌6例及肺癌患者自身正常肺组织8例。每例随机切3个蜡块,HE染色,每张切片随机取3-4个视野,用Image-Pro图像分析软件测试核及细胞,按体视学公式计算以细胞为参照空间时肺癌细胞核的体积密度(Vvn)、表面积密度(Svn)、表面积与体积比(Rsv,n)、数密度(Nvn)、核浆比(Rnp)、平均体积(vn)、平均表面积(sn)、平均自由程(n)及以核为参照系时核仁的数密度(Nvn2)。 结果:Vvn、Svn、Rsv,n、Nvn、vn、sn、Rnp、n、Nvn2均值分别为0.3304、0.2628m-1、0.7838m-1、0.0013m-3、383.7758m3、272.5568m2、0.9242、14.4891m、0.0011m-3,分别是正常肺泡上皮细胞核的2.22倍、1.44倍、61.17%、75.42%、4.41倍、2.56倍、5.22倍、73.95%,其范围分别为0.1432～0.9196、0.0883m-1～1.0411m-1、0.5687m-1～1.1321m-1、0.0002m-3 ～0.0103m-3、89.5911m3～765.0668m3、101.4292m2 ～491.3904m2、0.1672～11.4342、0.3090m～38.8223m、0.0000m-3～0.0059m-3,变异系数分别为0.5257、0.7386、0.1254、1.4079、0.4033、0.2856、2.1030、0.5338、1.0728。 结论: 1. 肺小细 OBJECTIVE: To quantitatively reveal the three-dimensional morphological characteristics and changes of lung cancer cell nuclei using stereological methods. Methods Thirty cases of adenocarcinoma, 20 cases of squamous cell carcinoma, 8 cases of adenosquamous carcinoma, 6 cases of undifferentiated small cell carcinoma, 6 cases of undifferentiated large cell carcinoma and 8 cases of normal lung tissue of lung cancer were collected. Each case was randomly divided into three blocks, HE staining, each section randomly taken 3-4 fields, with Image-Pro image analysis software to test the nucleus and cells, according to the stereological formula to calculate the cells as a reference space when the lung cancer nuclei Vvn, Svn, Rsv, n, Nvn, Rnp, Vn, Sn, mean free path (n) and the number density of nucleolus (Nvn2) when using the reference system as nucleus. Results: The average values ​​of Vvn, Svn, Rsv, n, Nvn, vn, sn, Rnp, n and Nvn2 were 0.3304,0.2628m-1,0.7838m-1,0.0013m-3,383.7758m3,272.5568m2,0.9242,14.4891 m and 0.0011m-3 respectively, which were 2.22 times, 1.44 times, 61.17%, 75.42%, 4.41 times, 2.56 times, 5.22 times and 73.95% of the normal alveolar epithelial cells respectively. The range of them was 0.1432-0.9196,0.0883m-1 To 1.0411m-1, 0.5687m-1 to 1.1321m-1, 0.0002m-3 to 0.0103m-3, 89.5911m3 to 765.0668m3, 101.4292m2 to 491.3904m2, 0.1667 to 11.4342, 0.3090m to 38.8223m, 0.0000m -3 ~ 0.0059m-3, the coefficient of variation were 0.5257,0.7386,0.1254,1.4079,0.4033,0.2856,2.1030,0.5338,1.0728. Conclusions: 1 small lungs