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目的观察司坦唑醇(ST)对注射促性腺激素释放激素类似物(GnRHa)的青春期SD大鼠长骨生长的影响,在生长板水平观察ST是否能促进大鼠长骨的生长/成熟平衡。方法90只雌鼠3周龄末时断乳按随机分组(同窝)设计分7个量效组:5 000μg/100 g、200μg/100 g、100μg/100 g、50μg/100 g、25μg/100 g、溶剂对照组、空白对照组(每组6只),分为8个时效组(每组6只),并开始肌注GnRHa制剂曲普瑞林,每2周1次,共2次,在第2次注射GnRHa3 d(D1)后开始ST干预处理。所有大鼠于D14处死,观察大鼠体格变化,并留取胫骨生长板进行HE染色及增殖细胞核抗原(PCNA)免疫组织化学半定量分析;双抗夹心ELISA法测血清胰岛素样生长因子-1(IGF-1)水平。结果1.随ST水平增加,大鼠体质量、体长、胫骨长逐渐增加,并以5 000μg/100 g组最明显(Pa<0.05);延长ST作用时间,大鼠体质量、体长、胫骨长逐渐增加,并以13 d组最明显(Pa<0.05)。2.随ST水平增加,生长板总宽度、增殖区宽度、肥大区宽度、增殖区构成比和PCNA阳性细胞数逐渐增加,在200μg/100 g组达最大值,5 000μg/100 g组以上指标均下降。延长ST作用时间,生长板总宽度、增殖区宽度、肥大区宽度、增殖区构成比和PCNA阳性细胞数逐渐增加,10 d组达最大值,13 d组以上指标均下降。3.ST作用后血清IGF-1水平未发生明显改变。结论司坦唑醇可促进GnRHa处理后青春期大鼠生长,在一定的剂量和疗程内,加速生长板老化的效应不明显。ST促进生长的作用不依赖于循环生长激素/IGF-1轴内分泌调控。
Objective To observe the effect of stanozolol (ST) on the growth of long bones of adolescent SD rats injected with gonadotropin-releasing hormone analogue (GnRHa). To observe whether ST can promote the growth / maturation of long bones in rats. Methods 90 females at the end of 3 weeks of age were randomly divided into 5 groups (5 000 μg / 100 g, 200 μg / 100 g, 100 μg / 100 g, 50 μg / 100 g, 25 μg / 100 g, solvent control group and blank control group (6 rats in each group) were divided into 8 aging groups (6 in each group), and started triptorelin GnRHa preparation intramuscularly once every 2 weeks for 2 times , ST intervention was started after the second GnRHa3 d (D1) injection. All the rats were sacrificed at D14, and the changes of their physical appearance were observed. The tibial growth plates were harvested for HE staining and PCNA immunohistochemical semiquantitative analysis. The levels of serum insulin-like growth factor-1 IGF-1) levels. Body weight, body length and tibia length increased gradually with the increase of ST level, and were most obvious in 5 000 μg / 100 g group (Pa <0.05); prolonged ST action time, body weight, body length, The tibia length increased gradually, and was most obvious in 13 d group (Pa <0.05). With the increase of ST level, the total width of growth plate, the width of proliferative zone, the width of hypertrophy zone, the proportion of proliferative zone and the number of PCNA positive cells increased gradually, reaching the maximum value in 200μg / 100g group and above 5 000μg / 100g All declined. Prolonging the duration of ST, the total width of growth plate, the width of proliferative zone, the width of hypertrophic zone, the proportion of proliferative zone and the number of PCNA positive cells increased gradually, reaching the maximum at 10 d. 3.ST serum IGF-1 levels did not change significantly. Conclusion Stanozolol can promote the growth of adolescent rats after GnRHa treatment, and the effect of accelerating the growth of growth plate is not obvious at a certain dose and duration of treatment. The role of ST-promoting growth is independent of the regulation of circulating growth hormone / IGF-1 axis endocrine.