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目的:研究和厚朴酚、青蒿素及二者联合应用在体外能否抑制人鼻咽癌CNE-2细胞的生长以及对其细胞凋亡的影响。方法:MTT法检测和厚朴酚、青蒿素单独或联合对细胞生长的抑制作用,以IC50评判抗肿瘤的效果,应用金氏公式进行联合用药的结果分析;DAPI染色观察细胞的凋亡形态,流式细胞仪检测和厚朴酚、青蒿素单独或联合作用对CNE-2凋亡率的影响。结果:和厚朴酚能显著抑制CNE-2细胞的增殖,并呈剂量依赖关系,其半数抑制浓度(IC50)为8.58 mg.L-1;青蒿素对CNE-2细胞生长没有明显的抑制作用,半数抑制浓度(IC50)为62.24 mg.L-1;和厚朴酚与青蒿素联合应用对CNE-2细胞的增殖抑制作用优于各单药组,q>1.15。DAPI染色可观察到和厚朴酚与青蒿素联合组核碎裂、凋亡小体,而单药组则不明显;凋亡分析显示联合用药组作用48 h细胞凋亡率显著高于各单药组。结论:和厚朴酚单独能够杀伤CNE-2细胞,并呈量-效关系,青蒿素单独作用不杀伤CNE-2细胞,两者联合应用对CNE-2细胞具有协同杀伤作用。
OBJECTIVE: To investigate whether honokiol, artemisinin and their combination can inhibit the growth of human nasopharyngeal carcinoma CNE-2 cells in vitro and its effect on apoptosis. Methods: The inhibitory effects of honokiol and artemisinin alone or in combination on the cell growth were detected by MTT assay. The anti-tumor effect was evaluated by IC50. The results of combination therapy were analyzed by Kim’s formula. The apoptotic morphology was observed by DAPI staining The effects of honokiol and artemisinin alone or in combination on the apoptosis rate of CNE-2 were detected by flow cytometry. RESULTS: Honokiol significantly inhibited the proliferation of CNE-2 cells in a dose-dependent manner with IC50 of 8.58 mg.L-1. Artemisinin had no significant inhibitory effect on the growth of CNE-2 cells (IC50) was 62.24 mg.L-1. The inhibitory effect of honokiol combined with artemisinin on CNE-2 cells was better than that of single drug group (q> 1.15). DAPI staining was observed in the combination of honokiol and artemisinin nuclear fragmentation and apoptotic bodies, while the single drug group is not obvious; apoptosis analysis showed that 48 h apoptosis rate was significantly higher than the combination group Monotherapy group. CONCLUSION: Honokinol can kill CNE-2 cells alone and in a dose-effect relationship. Artemisinin alone can not kill CNE-2 cells, and the combination of the two can have a synergistic cytotoxic effect on CNE-2 cells.