论文部分内容阅读
目的:分析和比较红细胞分布宽度(RDW)变化对电阻抗法血小板计数的影响。方法:搜集70fl≤红细胞平均体积(MCV)≤100fl,血小板(PLT)≥100×109/L,血小板平均体积(MPV)≤12fl,血小板平均分布宽度(PDW)≤17.2%的住院血液分析标本524例。以血小板手工计数法作为金标准,同时用电阻抗法和光学法检测血小板数量,分为:82fl≤MCV≤100fl且RDW<14.5%;82fl≤MCV≤100fl且RDW≥14.5%;70fl≤MCV<82fl且RDW<14.5%;70fl≤MCV<82fl且RDW≥14.5%四组,前两组和后两组分别用SPSS11.5进行配对t检验分析。结果:配对t检验分析显示,70fl≤MCV≤100fl范围内:当RDW<14.5%时,电阻抗法,光学法和手工计数法血小板计数差异均无统计学意义(P>0.05);当RDW≥14.5%时,电阻抗法和手工计数法血小板计数差异有统计学意义(P<0.05),光学法和手工计数法血小板计数差异无统计学意义(P>0.05),电阻抗法和光学法血小板计数差异有统计学意义(P<0.05)。结论:70fl≤MCV≤100fl范围,电阻抗法和光学法血小板计数差异受RDW影响而非MCV。血液分析检测中,70fl≤MCV≤100fl而RDW≥14.5%时,应当考虑用光学法复查血小板计数。
OBJECTIVE: To analyze and compare the influence of the change of RDW on the platelet count of electrical impedance method. Methods: Blood samples for in-hospital analysis of 70fl≤ MCFL≤100fl, PLT≥100 × 109 / L, MPV≤12fl and PDW≤17.2% were collected. example. Platelet count method as the gold standard, while the electrical impedance spectroscopy and optical detection of platelet count, divided into: 82fl≤MCV≤100fl and RDW <14.5%; 82fl≤MCV≤100fl and RDW≥14.5%; 70fl≤MCV < 82fl and RDW <14.5%; 70fl≤MCV <82fl and RDW≥14.5%. The first two groups and the latter two groups were analyzed by SPSS11.5 paired t-test. Results: Paired t-test showed that within the range of 70fl≤MCV≤100fl, there was no significant difference in the impedance method, optical method and manual counting platelet count when the RDW was less than 14.5% (P> 0.05) (P <0.05). There was no significant difference in the platelet counts between the optical method and the manual counting method (P> 0.05). The electrical impedance method and the optical platelet count The difference was statistically significant (P <0.05). Conclusion: The range of 70fl≤MCV≤100fl, the difference between electrical impedance method and optical method platelet count is affected by RDW but not MCV. In blood test, 70fl≤MCV≤100fl and RDW≥14.5%, we should consider using optical method to check the platelet count.