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用有机合成或酶促合成的AGDCGG为受体,以过量的pDAGp为供体,应用T_4RNA连接酶,成功地合成了酵母丙氨酸转移核糖核酸(tRNA_y~(Ala))的双氢尿核苷(D)环区九核苷九磷酸AGDCGGDAGp.在脱去3’-端磷酸基团后,得九核苷八磷酸.产物经序列分析证明,与AGDCGGDAG完全符合.本文也进行了相应于D环区九核苷酸的组成片段AG,AGDC,GG,GGp和DAG的5’-磷酸化和它们之间连接的条件探索,发现了3’-DMP和DAG的5’-磷酸化需在5～10℃下进行,可获近定量的产率. AGDCGGDAGp已用于tRNA_y~(Ala)的全分子合成.
Using AGDCGG synthesized by organic synthesis or enzymatic synthesis as acceptor and excess pDAGp as donor, dihydromyurin of yeast alanine transfer ribonucleic acid (tRNA_y ~ (Ala)) was successfully synthesized using T_4 RNA ligase (D) Ring region of nine nucleotide aglycone AGDCGGDAGp.After removing the 3’-terminal phosphate group, the nine nucleoside octaphosphoric acid was obtained.The product was confirmed by sequence analysis and AGDCGGDAG completely consistent with this article also carried out corresponding to the D ring The 5’-phosphorylation of AG, AGDC, GG, GGp and DAG, and the connection between them were investigated. It was found that the 5’-phosphorylation of 3’-DMP and DAG should be between 5 ~ Nearly a quantitative yield was obtained at 10 ° C. AGDCGGDAGp has been used for all-molecule synthesis of tRNA_y ~ (Ala).