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阻断大鼠双侧椎动脉和颈总动脉导致急性前脑缺血。在缺血30或60分钟后,立即解除颈总动脉夹闭状态,使再灌流4小时,记录活存率及实验前、后颅顶硬膜外脑电图;测定再灌流后脑组织的水、钠、钾含量,同时取材作光镜和电镜形态学观察。结果:缺血30分钟再灌流组动物表现有明显的脑水肿和异常脑电图改变;形态结构出现一定程度的不可逆损伤;有30％的死亡率。缺血60分钟再灌流组则出现更加严重的脑水肿和异常脑电图;形态结构呈现广泛的不可逆损伤,死亡率达83.3％。
Blocking bilateral bilateral vertebral and common carotid arteries in rats leads to acute forebrain ischemia. In 30 or 60 minutes after ischemia, immediately remove the common carotid artery occlusion, reperfusion 4 hours, record the survival rate and before and after the experiment, after epidural EEG; determination of brain tissue after reperfusion water, Sodium, potassium content, taken at the same time for light and electron microscopy Morphological observation. Results: In the 30 min ischemia reperfusion group, the animals showed obvious cerebral edema and abnormal EEG changes; the morphological structure showed a certain degree of irreversible damage; and 30% mortality rate. Severe cerebral edema and abnormal EEG appeared after ischemia 60 minutes in reperfusion group. The morphological structure showed extensive irreversible damage with a mortality rate of 83.3%.