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淋巴细胞杂交瘤技术作为一种新的生物工程技术,已在生物学和医学领域内得到广泛的应用。我们继建立产生抗北京鸭红细胞单克隆抗体的杂交瘤细胞系CBH-1后,又建立了一株产生抗北京鸭免疫球蛋白(I_g)单克隆抗体的杂交瘤细胞系,命名为CBH-2。这种杂交瘤细胞经体外培养,能稳定地分泌抗北京鸭I_g的单克隆抗体。此单克隆抗体在以北京鸭抗体进行放射免疫和酶标等测定时,可用作第二抗体。现将主要结果简报于下。北京鸭I_g抗原的制备及免疫:从北京鸭颈动脉采血,分离血清,用50%和33%硫酸铵沉淀法提取二次。每只Balb/cJ小鼠腹腔免疫剂量为0.3毫克,共免疫三
As a new bioengineering technique, lymphocyte hybridoma technology has been widely used in biology and medicine. Following the establishment of CBH-1, a hybridoma cell line that produces anti-Pekin duck monoclonal antibody, a hybridoma cell line producing anti-Peking duck immunoglobulin (I_g) monoclonal antibody was constructed and named as CBH-2 . This hybridoma cells cultured in vitro, can stably secrete anti-Beijing duck I_g monoclonal antibody. This monoclonal antibody can be used as a secondary antibody when it is subjected to radioimmunoassay and enzymatic assay using Beijing duck antibody. Now the main results briefed below. Preparation and Immunization of Beijing Duck I_g Antigen: Blood was collected from Beijing duck carotid arteries and serum was separated and extracted twice with 50% and 33% ammonium sulfate precipitation. Each Balb / cJ mice intraperitoneal immunization dose of 0.3 mg, co-immunization three