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[目的]研究筛查胎儿缺陷中孕期血清标本在-20℃存储条件下对检测结果的影响。[方法]随机抽取2012年1月—2014年12月收集的50例血清标本为研究对象,采用全自动时间分辨免疫分析系统检测并比较甲胎蛋白(AFP)、雌三醇(u E3)、游离人绒毛膜促性腺激素(free-β-HCG)的原始水平,以及-20℃条件下分别保存1年、2年的复查水平。[结果]血清标本AFP、u E3、free-β-h CG原始水平分别为(33.00±9.45)U/m L、(28.07±7.48)nmol/L、(4.06±1.52)ng/m L,-20℃保存1年后复查水平分别为(33.34±9.65)U/m L、(28.48±7.79)nmol/L、(3.99±1.60)ng/m L,保存2年后复查水平分别为(33.63±9.50)U/m L、(27.48±7.72)nmol/L、(4.77±1.66)ng/m L,同原始水平比,后两年水平差异均无统计学意义(P>0.05)。2年以前、-20℃保存1年、2年后血清筛查,胎儿缺陷临床检出率均为2.0%(1/50),检出者为同一份样品。[结论]贮存温度设置为-20℃对产前血清学标本的复检影响甚小,-20℃长期保存血清学筛查标本的方法可以接受。
[Objective] To study the effect of screening serum samples from preimplantation fetuses during pregnancy at -20 ℃ on the detection results. [Methods] Fifty serum samples collected from January 2012 to December 2014 were randomly selected for the study. AFP, uE3, Free chorionic gonadotropin (free-β-HCG) of the original level, and -20 ℃ conditions were stored for 1 year, 2 years review level. [Results] The original levels of AFP, uE3 and free-β-h CG in serum were (33.00 ± 9.45) U / m L, (28.07 ± 7.48) nmol / L, and 4.06 ± 1.52 ng / After 1 year of storage at 20 ℃, the levels of reviewing were 33.34 ± 9.65 U / m L, 28.48 ± 7.79 nmol / L and 3.99 ± 1.60 ng / m L, respectively. After 2 years of storage, the levels of reviewing were 33.63 ± 9.50) U / m L, (27.48 ± 7.72) nmol / L and (4.77 ± 1.66) ng / m L respectively. There was no significant difference between the two groups (P> 0.05). Two years ago, -20 ℃ preserved for 1 year, 2 years after the serum screening, the clinical detection rate of fetal defects were 2.0% (1/50), the same samples were detected. [Conclusion] Setting the storage temperature at -20 ℃ has little effect on the retesting of prenatal serological samples, and the method of long-term preservation of serological screening samples at -20 ℃ is acceptable.