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三株病毒用不同方法制备的疫苗，免疫家兔后都能诱导产生免疫荧光抗体（IF）。其中Z10和L99株用细胞冻融以β-丙内酯灭活制备的疫苗还可以测到HI抗体。以β-丙内酯灭活的疫苗所产生的中和抗体，以Z10株最高（1：80～1：160），L99株较低（1：10），而LR1株未测出。但三株病毒感染的细胞培养上清液，用两种灭活剂均未产生HI或NT抗体。
Three viruses prepared by different methods of vaccine, immunized rabbits can induce the production of immunofluorescence antibodies (IF). Among them, HI antibody could be detected in the vaccine prepared by inactivation of β-propiolactone by freeze-thaw in Z10 and L99 strains. Neutralizing antibodies produced by vaccines inactivated by beta-propiolactone showed the highest (1:80 to 1: 160) Z10 strain, the lower L99 strain (1:10), and the absence of the LR1 strain. However, none of the three virus-infected cell culture supernatants produced HI or NT antibodies with both inactivators.