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目的研究血管内皮细胞生长因子（ｖａｓｃｕｌａｒ ｅｎｄｏｔｈｅｌｉａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ， ＶＥＧＦ）与乙型肝炎（乙肝）分级（Ｇ）和分期（Ｓ）的关系。方法２５０例乙肝患者肝活检标本用ＶＥＧＦ单克隆抗体进行免疫组织化学（Ｓ－Ｐ法）染色。结果ＶＥＧＦ在肝组织表达有胞浆型、膜窦型及窦内皮细胞型；ＶＥＧＦ主要由肝细胞分泌，其次在肝窦内皮细胞、贮脂细胞、成肌纤维细胞及单核细胞也示阳性。无明显病变的血管内皮细胞及肝细胞ＶＥＧＦ染色阴性。提示随分级增高ＶＥＧＦ表达增强，其间差异有极显著意义（ｘ~２＝９０．４３，Ｐ＜０．０１）；而Ｓ低分值者ＶＥＧＦ多有阳性表达，分值较高者多为强阳性表达，两者差异明显（ｘ~２＝１１５７４，Ｐ＜０．０１）。结论ＶＥＧＦ表达与乙肝Ｇ和Ｓ相关。
Objective To study the relationship between vascular endothelial growth factor (VEGF) and hepatitis B (G) and staging (S). Methods 250 liver biopsy specimens from patients with hepatitis B were stained with VEGF monoclonal antibody by immunohistochemistry (S-P method). Results The expression of VEGF in the liver tissue was cytoplasm, membrane sinus and sinus endothelial cell type. VEGF was mainly secreted by hepatocytes, followed by sinusoidal endothelial cells, fat storage cells, myofibroblasts and monocytes. Vascular endothelial cells and hepatocytes without obvious pathological changes were negative for VEGF staining. It suggested that the expression of VEGF increased with the increase of grading, the difference was significant (x ~ 2 = 90.43, P <0.01); while the low score of VEGF was more positive expression, Positive expression, the difference was significant (x ~ 2 = 11574, P <0.01). Conclusion The expression of VEGF is related to G and S of hepatitis B virus.