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为建立小叶兜兰的繁育技术体系,本研究通过无菌播种的方法,辅以TTC生活力测定等方法,比较了小叶兜兰种子在授粉后不同发育时期和培养条件下的萌发率,对小叶兜兰种胚的发育过程进行了显微观察,探讨种胚发育程度与萌发的关系。结果表明,小叶兜兰种胚的发育阶段对萌发的影响最大,授粉后255 d的种子萌发率最高(90.71%),该阶段种子仍呈白色但微干燥,种胚刚发育至球形胚阶段,胚柄尚存。1/4MS和1/2MS为小叶兜兰适宜的基本培养基,添加100 mg·L-1的土豆汁对小叶兜兰的无菌萌发有良好的促进作用。
In order to establish a breeding technology system of Paphiopedia oryzae, this study compared the germination rate of Paphiola germplasm at different developmental stages and culture conditions after pollination by aseptic sowing method combined with the determination of viability of TTC, Paphiopedilum embryo development was microscopically observed to explore the relationship between embryo development and germination. The results showed that the developmental stage of Porphyra orbiculata embryo had the greatest influence on the germination. The seed germination rate was the highest at 255 d after pollination (90.71%). The seed was white but slightly dry at this stage, Scabs surviving. 1 / 4MS and 1 / 2MS were the suitable basic medium of Paphiopedia scoparia. The addition of 100 mg · L -1 potato juice could promote the germination of Paphioora argentea.