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目的研究跨膜型血管内皮生长因子受体1(Flt-1)在子痫前期患者胎盘组织中的表达变化及其意义。方法采用RT-PCR方法检测20例子痫前期患者(观察组)胎盘组织中Flt-1mRNA的相对表达量[以Flt-1与β肌动蛋白(β-actin)电泳条带吸光度(A)值的比值表示],并以20例正常孕妇(对照组)为对照;采用蛋白印迹(westernblot)法检测和比较两组各18例胎盘组织中Flt-1的蛋白相对表达量[其余4例标本因蛋白提取不理想而废弃,以Flt-1与磷酸甘油醛脱氢酶(GAPDH)电泳条带A值的比值表示]。结果两组孕妇胎盘组织中均有Flt-1的表达,观察组孕妇胎盘组织Flt-1mRNA相对表达量为2·25±0·19,对照组为1·23±0·29,两组比较,差异有统计学意义(P<0·05);观察组孕妇胎盘组织Flt-1蛋白相对表达量为2·67±1·19,对照组为0·94±0·51,两组比较,差异也有统计学意义(P<0·05)。结论Flt-1可能参与了子痫前期的病理生理过程。
Objective To investigate the expression and significance of transmembrane-type vascular endothelial growth factor receptor 1 (Flt-1) in placenta of preeclampsia patients. Methods RT-PCR was used to detect the relative expression of Flt-1mRNA in placenta of 20 preeclampsia patients (observation group). The absorbance (A) of Flt-1 and β-actin bands Ratio of 20] normal pregnant women (control group) as a control; Western blot (western blot) was used to detect and compare the relative expression of Flt-1 protein in 18 placenta tissues of the two groups [the remaining 4 specimens due to protein Extraction is not ideal and abandoned, with Flt-1 and glyceraldehyde phosphate dehydrogenase (GAPDH) electrophoresis band A value ratio of the value]. Results The expression of Flt-1 in placenta of both groups was significantly higher than that in the control group (P <0.05). The relative expression of Flt-1 mRNA in the placenta was 2.25 ± 0.19 in the observation group and 1.23 ± 0.29 in the control group (P <0.05). The relative expression level of Flt-1 protein in placenta of observation group was 2.67 ± 1.19 in control group and 0.94 ± 0.51 in control group, the difference was statistically significant There was also statistical significance (P <0.05). Conclusion Flt-1 may be involved in the pathophysiological process of preeclampsia.