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Home-made alkaline phosphatase (HMAKP) applied to indirect ELISA techniqueis reported for the first time. HMAKP and Sigma (RZ=3) horseradish peroxidase (HRP)were coupled respectively with goat anti-human-IgG by one step method of glutaraldhyde,
HMAKP and Sigma (RZ = 3) horseradish peroxidase (HRP) were coupled respectively with goat anti-human-IgG by one step method of glutaraldhyde,