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背景:生物力学环境的异常及一氧化氮过度表达是椎间盘退变进程中重要的影响因素。目的:探讨静水压下芹菜素对体外单层培养的人髓核细胞凋亡率及基质代谢的影响。方法:在静水压加载系统中对体外单层培养的人髓核细胞(传4代)施以0.1～3.0MPa的静压,加压2h,同时使用芹菜素和一氧化氮供体对髓核细胞进行干预,分析不同静水压下芹菜素对髓核细胞凋亡率、基质金属蛋白酶及基质表达的影响。结果与结论:在0.3MPa静水压下,芹菜素能改善由于高浓度一氧化氮引起的髓核细胞功能抑制状态,降低基质金属蛋白酶3过表达,恢复基质金属蛋白酶3与其抑制因子1之间的平衡;而在3MPa静水压下,芹菜素能降低一氧化氮引起的髓核细胞高凋亡,并在一定程度上缓解基质金属蛋白酶3与其抑制因子1之间的不平衡。说明芹菜素能够对髓核细胞的功能状态、凋亡率,对基质金属蛋白酶3及其抑制因子1平衡产生影响,而且这种影响在不同的静水压环境下有所不同。
BACKGROUND: Abnormal biomechanical environments and overexpression of nitric oxide are important factors in the process of disc degeneration. Objective: To investigate the effect of apigenin on apoptosis rate and matrix metabolism of human nucleus pulposus cultured in vitro under hydrostatic pressure. Methods: Human mononuclear cells (IVF) cultured in vitro were hydrostatically compressed at 0.1 ~ 3.0 MPa for 2 h. The apigenin and nitric oxide donor The effects of apigenin on the apoptosis rate of nucleus pulposus cells, matrix metalloproteinase and matrix in different hydrostatic pressure were analyzed. RESULTS AND CONCLUSION: At 0.3MPa hydrostatic pressure, apigenin can improve the inhibitory function of nucleus pulposus cells induced by high concentration of nitric oxide, decrease the expression of matrix metalloproteinase 3 and restore the expression of matrix metalloproteinase 3 and its inhibitory factor 1 ; At 3MPa hydrostatic pressure, apigenin can reduce the high apoptosis of nucleus pulposus cells induced by nitric oxide, and to a certain extent, alleviate the imbalance between matrix metalloproteinase 3 and its inhibitory factor 1. It indicates that apigenin can affect the functional status and apoptosis rate of nucleus pulposus cells and the balance of matrix metalloproteinase 3 and its inhibitory factor 1, and this effect is different under different hydrostatic conditions.