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目的探讨白藜芦醇对慢性前列腺炎大鼠模型的治疗机制。方法 50只体质量(150±15)g的雄性SD大鼠随机分为5组:阴性对照组和模型假性对照组、白藜芦醇低、中、高剂量模型组。模型组分别在第0、30天于大鼠皮下注射大鼠前列腺蛋白提纯液和弗氏完全佐剂的混悬液,同时腹腔注射百白破疫苗造模;阴性对照组以生理盐水注射参照。第45天开始,白藜芦醇低、中和高剂量模型组每日按照白藜芦醇4 mg/kg、20 mg/kg、40mg/kg灌胃给药,阴性对照组和模型假性对照组以生理盐水灌胃。第55天后处死大鼠取左侧前列腺组织,10%甲醛溶液固定,做石蜡包埋切片,HE染色,镜下观察大鼠前列腺病理形态的改变。右侧前列腺打成组织匀浆,免疫组化ELISA法检测白介素-1β(IL-1β)、IL-6、IL-8、IL-10和肿瘤坏死因子(TNF-α)的含量。计量数据用(x±s),统计分析用t检验。结果白藜芦醇高、中剂量组与模型假性对照组比较,前列腺间质中炎细胞数量显著降低,成纤维细胞数量轻度下降,腺体数量与腺腔面积明显升高;前列腺匀浆中IL-1β、IL-8、TNF-α明显降低,IL-6、IL-10含量显著上升(P<0.01)。结论白藜芦醇对慢性免疫性前列腺炎大鼠模型的治疗是通过降低前列腺内促炎性细胞因子IL-1?、IL-8、TNF-α,提高抑炎性细胞因子IL-6、1L-10来减轻炎细胞浸润,抑制纤维组织增生完成。
Objective To investigate the therapeutic mechanism of resveratrol in chronic prostatitis rat model. Methods Fifty male Sprague-Dawley (SD) rats weighing 150 ± 15 g were randomly divided into 5 groups: negative control group, model control group and low, medium and high dose resveratrol model groups. The rats in model group were subcutaneously injected with a suspension of prostatic protein and Freund’s complete adjuvant in rats on the 0th and 30th day, respectively. At the same time, the model mice were injected intraperitoneally with diphtheriae vaccine. The negative control group was injected with normal saline. On the 45th day, the resveratrol low, middle and high dose model groups were administered with resveratrol 4 mg / kg, 20 mg / kg and 40 mg / kg orally daily, and the negative control group and the model pseudo-control Group with normal saline gavage. Left after the first 55 days, the rats were sacrificed, the left prostate tissue was fixed, fixed in 10% formaldehyde solution, paraffin-embedded sections were made and stained with hematoxylin and eosin. The pathological changes of the prostate were observed under microscope. The right prostate was labeled with homogenate and the contents of IL-1β, IL-6, IL-8, IL-10 and tumor necrosis factor (TNF-α) were detected by immunohistochemistry. Measurement data with (x ± s), statistical analysis using t test. Results Compared with the model control group, the number of inflammatory cells in prostate stroma was significantly decreased, the number of fibroblasts slightly decreased, the number of glands and the glandular cavity area were significantly increased. Prostatic hyperplasia The levels of IL-1β, IL-8 and TNF-α were significantly decreased, and the levels of IL-6 and IL-10 were significantly increased (P <0.01). Conclusion The therapeutic effect of resveratrol on chronic unpredictable prostatitis in rats is that the proinflammatory cytokines IL-1, IL-8 and TNF-α in the prostate are decreased and IL-6 and IL- -10 to reduce inflammatory cell infiltration, inhibit the completion of fibrous tissue hyperplasia.