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为了解抗病毒核苷类似物拉米夫定(3TC)处理的Hep2.2.15细胞干扰素(IFN)抗病毒基因表达谱以及3TC对IFN信号转导途径的影响而开展此项研究。具体方法:将培养的Hep2.2.15细胞用3TC处理,再以IFNα刺激6h,分离细胞总RNA,经逆转录、32P标记后与尼龙膜上的探针基因芯片进行分子杂交—
This study was conducted to understand the antiviral gene expression profile of Hep2.2.15 interferon (IFN) antiviral nucleoside analogue lamivudine (3TC) and the effect of 3TC on the IFN signaling pathway. Specific methods: The cultured Hep2.2.15 cells treated with 3TC, then stimulated with IFNα 6h, total cellular RNA was isolated by reverse transcription, 32P labeled with nylon membrane probe gene chip molecular hybridization -