目的获得７型腺病毒疫苗株（Ａｄ７ｖ）７６５－８７ｍｕ核苷酸序列，分析该区段的基因结构和功能。方法从Ａｄ７疫苗株克隆出６８－８７ｍｕ核苷酸片段，应用Ｓａｎｇｅｒ双脱氧法进行核苷酸序列分析。结果Ａｄ７ｖ７６５～８７ｍｕ核苷酸序列长度为３５５７ｂｐ，推测此片段编码Ｅ３区１２１ｋＤ、１９２ｋＤ、２０１ｋＤ、２０５ｋＤ、１０３ｋＤ和１５２ｋＤ蛋白的一部分。所编码的６个蛋白与Ａｄ７原型株（Ａｄ７ｐ）和Ａｄ７ｈ（１９８６年以来南美流行的毒株）核苷酸高度同源（大于９７２％）。１６１ｋＤ蛋白由于缺失一个核苷酸，造成移码突变，编码提前终止。编码７７ｋＤ蛋白的ＯＲＦ由于缺失一段核苷酸而提前终止编码。结论这一结果为阐明Ａｄ７的基因结构与功能并为构建Ａｄ７载体时对Ｅ３区缺失提供依据 Objective To obtain the nucleotide sequence of 765-87mu of adenovirus type 7 adenovirus vaccine (Ad7v) and to analyze the gene structure and function of this segment. Methods The 68-87mu nucleotide fragment was cloned from the Ad7 vaccine strain and the nucleotide sequence was analyzed by Sanger dideoxy method. Results The nucleotide sequence of Ad7v765 ~ 87mu was 3557bp, suggesting that this fragment encodes a part of 12  1 kD, 19  2kD, 20  1kD, 20  5kD, 10  3kD and 15  2kD proteins in E3 region. The six proteins encoded are highly homologous (greater than 97.2%) nucleotides to the Ad7p and Ad7h strains (strains of South American origin that have been circulating since 1986). 16  1kD protein due to the deletion of a nucleotide, resulting in frameshift mutations, coding early termination. The ORF encoding a 7. 7 kD protein terminates coding prematurely due to the deletion of a stretch of nucleotides. Conclusions This result is to elucidate the genetic structure and function of Ad7 and provide a basis for deletion of E3 region for the construction of Ad7 vector