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采用光镜及电镜双标记技术，对大鼠内侧视前区（ＭＰＯ）神经元发出的投射到脑桥Ｂａｒｉｎｇｔｏｎ核的轴突与其内投射到骶髓的神经元的胞体和树突之间的突触关系进行了探讨。将顺行追踪剂结合生物素的葡聚糖胺（ＢＤＡ）注射于ＭＰＯ用来标记它发出的轴突终末；将逆行追踪剂辣根过氧化物酶（ＨＲＰ）注射于脊髓腰骶段，以此标记Ｂａｒｉｎｇｔｏｎ核内投射至腰骶髓的神经元。光镜观察发现Ｂａｒｉｎｇｔｏｎ核内含有大量ＢＤＡ顺行标记的终末及ＨＲＰ逆行标记的树突和胞体，电镜观察确证了ＢＤＡ标记的轴突终末与ＨＲＰ标记的树突和胞体间存在直接的突触联系，且均为对称性突触。此结果提示ＭＰＯ可能通过这一直接的纤维联系调节排尿反射活动。
Using light and electron microscopy double labeling, the synapses between the neurons of the medial preoptic area (MPO) in the rat projecting to the Barington nucleus of the pons and the soma and dendrites of neurons projecting into the sacral spinal cord The relationship was explored. Injecting dextran amine (BDA), a biotinylated tranexamic acid tracer, into MPO to label the terminal axons it emits; injecting retrograde tracer horseradish peroxidase (HRP) into the lumbosacral segment of the spinal cord, In this way, neurons projecting to the lumbosacral spinal cord in the Barington nucleus are labeled. Light microscopy showed that the Barington nucleus contained a large number of terminal dendritic markers of BDA and HRP retrogradely labeled dendrites and cell bodies. Electron microscopy confirmed the direct projection of BDA-labeled axon terminals and HRP-labeled dendrites and cell bodies Touch, and are symmetrical synapses. This result suggests that MPO may regulate urination reflex activities through this direct fiber connection.