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目的 研究热应激反应对感染性休克大鼠肺损伤的影响。方法 Ｗｉｓｔａｒ大鼠２４ 只随机分为三组。分别经肺动脉注射ＮＳ０．５ｍ ｌ（Ⅰ组）或内毒素（ＬＰＳ）１５ｍ ｇ·ｋｇ－ １ （Ⅱ组）或热应激处理１５ 分钟恢复２４ 小时后注射ＬＰＳ１５ｍ ｇ·ｋｇ－ １（Ⅲ组），然后取注射ＮＳ或ＬＰＳ前和后１、１．５、２、２．５、３、４ 小时末的动脉血分离血浆测定ＴＮＦ－α，同时用四导生理仪记录其平均肺动脉压（ｍ ＰＡＰ），平均动脉压（ＭＡＰ）、气道压（ＡＰ）、心率（ＨＲ）等血液动力学指标，并定期作血气分析测定动脉血氧分压（ＰａＯ２ ）。于４ 小时末活杀动物，取出肺脏称重，计算肺系数（ＬＣ）。结果 （１）Ⅱ、Ⅲ组动物注射ＬＰＳ后ＴＮＦ－α浓度明显升高，与Ⅰ组对比差异显著（Ｐ＜ ０．０５～０．０１），ＴＮＦ－α浓度于１．５～２ 小时达到峰值，２ 小时后开始下降，４ 小时基本恢复至基础值，其中Ⅲ组ＴＮＦ－α浓度明显低于Ⅱ组各相应时间点ＴＮＦ－α浓度值（Ｐ＜ ０．０５）；（２）Ⅱ、Ⅲ组动物于注射ＬＰＳ０．５ 小时后ＭＡＰ、ＰａＯ２ 开始明显下降，ｍ ＰＡＰ、ＡＰ、ＨＲ明显上升，与Ⅰ组比较差异显著（Ｐ＜ ０．０５～０．０１），Ⅱ组变化值较Ⅲ组相应时间点?
Objective To investigate the effect of heat stress on lung injury in septic shock rats. Methods Twenty-four Wistar rats were randomly divided into three groups. The mice were injected with 15 m g · kg -1 of NS (0.5 mg · L -1) or 15 mg · kg -1 (LPS) or heat shock for 15 minutes respectively. After 24 hours, LPS (15 m g · kg -1) , And then measured TNF-αin plasma before and after 1, 1.5, 2, 2.5, 3, 4 hours after injection of NS or LPS, and mean pulmonary arterial pressure PAP, MAP, AP, HR, etc were measured. PaO2 was periodically determined by blood gas analysis. Animals were sacrificed at the end of 4 hours, the lungs were removed and weighed to calculate the lung coefficient (LC). Results (1) The concentration of TNF-α in group Ⅱ and Ⅲ was significantly higher than that in group Ⅰ (P <0.05-0.01), and the concentration of TNF-α reached at 1.5 ~ 2 hours (P <0.05); (2) The concentration of TNF-α in group Ⅲ was significantly lower than that in group Ⅱ (P <0.05); (2) In group Ⅲ, MAP and PaO2 began to decrease significantly after 0.5 hour LPS injection, and m PAP, AP and HR increased significantly (P <0.05 ~ 0.01) Group corresponding time?