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目的:探讨粉尘螨滴剂舌下特异性免疫治疗(SLIT)对调节性T淋巴细胞水平的影响和意义。方法:选择粉尘螨滴剂舌下特异性免疫治疗满1年的轻度支气管哮喘患儿30例作为实验组(SLIT组),同时选择未经SLIT治疗的对粉尘螨过敏的轻度支气管哮喘患儿30例作为对照组(非SLIT组)。分离所有入选患儿的外周血淋巴细胞(PBMC),给予粉尘螨浸出液刺激后检测CD4+CD25+Foxp3+T淋巴细胞百分比和可分泌IL-10的CD4+CD25+Foxp3+T淋巴细胞百分比的变化。结果:①SLIT组和非SLIT组患儿调节性T淋巴细胞的数量无统计学差异;②SLIT组患儿调节性T淋巴细胞的功能显著高于非SLIT组,差异有统计学意义(P<0.05)。结论:舌下特异性免疫治疗有可能通过上调诱导性调节性T淋巴细胞的功能来诱导免疫耐受;诱导性调节性T淋巴细胞在舌下特异性免疫治疗中可能发挥着重要作用。
Objective: To investigate the effect and significance of sublingual immunotherapy (SLIT) on the level of regulatory T lymphocytes in mice. Methods: Thirty children with mild bronchial asthma who had been treated with dust mite drops for 1 year were selected as experimental group (SLIT group), and mild bronchial asthma who were allergic to dust mites without SLIT Thirty children as control group (non-SLIT group). Peripheral blood lymphocytes (PBMCs) were isolated from all infants and the percentage of CD4 + CD25 + Foxp3 + T lymphocytes and the percentage of CD4 + CD25 + Foxp3 + T lymphocytes secreting IL-10 after stimulation with Dust mite leachate . Results: ①There was no significant difference in the number of regulatory T lymphocytes between SLIT group and non-SLIT group. ② The function of regulatory T lymphocytes in SLIT group was significantly higher than that in non-SLIT group (P <0.05) . Conclusion: Sublingual immunotherapy may induce immune tolerance by upregulating the function of induced regulatory T lymphocytes. Inducible regulatory T lymphocytes may play an important role in sublingual immunotherapy.