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目的研究中国汉族人对氧磷酯酶1(PON1)活性及PON1Q/R192基因多态性与动脉粥样硬化性血栓性脑梗死(ATCI)发病的关系。方法采用紫外分光光度法和PCRRELP方法检测48例ATCI患者和55例相匹配的健康人血清PON1活性及PON1Q/R192基因多态性,同时测定血脂、血糖等指标,并进行统计学分析。结果ATCI组血清PON1活性为(74.41±18.85)μg/ml,低于对照组[(113.65±26.64)μg/ml,P<0.05];ATCI组与对照组等位基因频率分别为Q37%、43%;R63%、57%,2组间比较差异无显著性,2组间3种基因型的分布差异也无显著性。结论血清PON1活性降低可能是ATCI的独立危险因素,而PON1Q/R192基因多态性与中国汉族人ATCI的发生无关。
Objective To investigate the relationship between the activity of paraoxonase 1 (PON1) and the polymorphism of PON1Q / R192 gene and the incidence of atherothrombotic cerebral infarction (ATCI) in Chinese Han population. Methods The serum PON1 activity and PON1Q / R192 gene polymorphism in 48 patients with ATCI and 55 matched healthy individuals were detected by UV spectrophotometry and PCRRELP method. The levels of blood lipids, blood glucose and other indicators were also determined and statistically analyzed. Results The serum PON1 activity of ATCI group was (74.41 ± 18.85) μg / ml which was lower than that of the control group [(113.65 ± 26.64) μg / ml, P <0.05]. The allele frequencies of ATCI group and control group were Q37%, 43 %; R63%, 57%, no significant difference between the two groups, the distribution of the three genotypes between the two groups was also no significant difference. Conclusions The decreased serum PON1 activity may be an independent risk factor for ATCI. However, the polymorphism of PON1Q / R192 gene is not associated with the occurrence of ATCI in Chinese Han population.