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用成年云南山楂Crataegus scabrifolia(Franch)Rehd.树的无菌增殖芽苗作为生根试验材料。结果表明:接种在含生长素(IBA、NAA)0.01—1.0mg/l的MS/2培养基中,芽苗的平均生根率为50.4％;在高浓度(150—250mg/l)生长素溶液中浸泡芽苗基部30分钟,然后接种到无生长素的MS/2培养基中,其生根率为60.1％;用高浓度生长素液蘸芽苗基部的生根率为83.5％;芽苗在含IBA 1—5mg/l的培养基中培养2—4天,然后转入MS/2培养基中诱导生根,生根率可达92％以上,根伸长正常。黑暗条件明显抑制生根,每日16小时至24小时光照对芽苗生根有益。
Sterile multiplication sprouts from adult Crataegus scabrifolia (Franch) Rehd. Tree were used as rooting materials. The results showed that the average rooting rate of sprouts was 50.4% when inoculated in MS / 2 medium containing 0.01-1.0 mg / l of auxin (IBA, NAA). In the high concentration (150-250 mg / l) of auxin solution Soaking the base of the sprouts for 30 minutes and then inoculating to the auxin-free MS / 2 medium, the rooting rate is 60.1%; the rooting rate is 83.5% at the base of the sprouts treated with the high concentration of auxin solution; IBA 1-5mg / l medium for 2-4 days, and then transferred to MS / 2 medium to induce rooting, rooting rate of up to 92%, root elongation normal. Dark conditions significantly inhibited rooting, daily 16 hours to 24 hours of light on the bud seedling beneficial.