论文部分内容阅读
目的:建立HPLC同时测定乳癖消颗粒中芍药苷、三七皂苷R1、人参皂苷Rg1、连翘苷、哈巴俄苷、人参皂苷Rb1、去氢木香内酯7种成分的含量。方法:采用AlltimaC18色谱柱(4.6 mm×250 mm,5μm);乙腈-水为流动相,梯度洗脱,检测波长203 nm,柱温30℃,流速1.0 mL·min-1。结果:芍药苷、三七皂苷R1、人参皂苷Rg1、连翘苷、哈巴俄苷、人参皂苷Rb1、去氢木香内酯线性范围分别为3.906 25～125μg(r=0.999),9.375～300μg(r=0.999),37.5～1 200μg(r=1.000),2.187 5～70μg(r=0.999),3.75～120μg(r=0.999),25～800μg(r=0.999),0.625～20μg(r=1.000);平均加样回收率分别为97.95%(RSD 0.71%),99.05%(RSD 1.11%),100.70%(RSD 2.46%),102.74%(RSD 0.35%),103.06%(RSD 0.55%),98.25%(RSD 1.68%),100.16%(RSD 1.82%)。结论:本方法方便、稳定、可靠,可用于乳癖消颗粒的质量控制。
OBJECTIVE: To establish an HPLC method for the simultaneous determination of seven components, including paeoniflorin, notoginsenoside R1, ginsenoside Rg1, forsythin, harpagoside, ginsenoside Rb1 and dehydrobronolactone in Rupi Xiao granules. Methods: Alltima C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and water with a gradient of 203 nm. The column temperature was 30 ℃ and the flow rate was 1.0 mL · min-1. Results: The linear ranges of paeoniflorin, notoginsenoside R1, ginsenoside Rg1, forsythin, harpagoside, ginsenoside Rb1 and dehydrobrindolactone were 3.906 25 ~ 125μg (r = 0.999), 9.375 ~ 300μg (r = 0.999), 25 ~ 800μg (r = 0.999), 0.625 ~ 20μg (r = 1.999), 2.187 5 ~ 70μg (r = ). The average recoveries were 97.95% (RSD 0.71%), 99.05% (RSD 1.11%), 100.70% (RSD 2.46%), 102.74% (RSD 0.35%), 103.06% (RSD 0.55%), 98.25 % (RSD 1.68%), 100.16% (RSD 1.82%). Conclusion: The method is convenient, stable and reliable and can be used for the quality control of Rupixiao granule.