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目的:探讨川芎嗪(LGT)、左旋精氨酸(L-Arg)联合使用对肝缺血-再灌注损伤(HIRI)时肝细胞能量代谢的影响及其机制。方法:实验兔40只,建立肝缺血-再灌注模型后随机分4组:模型组,LGT组,L-Arg组和LGT+L-Arg组,每组10只。再灌注45 min时,分别检测肝组织内三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)含量、总腺苷酸量(TAN)、能荷(EC)、丙二醛浓度(MDA)、超氧化物歧化酶活性(SOD)、一氧化氮代谢产物(NO2-／NO3-)水平、血栓素B2(TXB2)、6-酮基-前列腺素F1α(6-keto-PGF1α)含量和TXB2／6-keto-PGF1α比值。结果:与模型组比较,LGT组、L-Arg组、LGT+L-Arg组肝组织内ATP、EC、NO2-／NO3-、6-keto-PGF1α含量及SOD活性均明显增高(P<0.05和P<0.01),MDA、TXB2含量及TXB2／6-keto-PGF1α比值均显著减少(P<0.05和P<0.01)。结论:LGT联用L-Arg可通过降低体内氧自由基水平、提高一氧化氮水平、纠正TXA2与PGI2的平衡,而改善缺血-再灌注损伤肝脏的能量代谢。
Objective: To investigate the effects of Ligustrazine (L-Arg) combined with Ligustrazine (LGT) on the energy metabolism of hepatocytes during hepatic ischemia-reperfusion injury (HIRI) and its mechanism. Methods: Forty rabbits were randomly divided into 4 groups: model group, LGT group, L-Arg group and LGT + L-Arg group, with 10 rats in each group. At 45 min after reperfusion, the levels of ATP, AMP, AMP, TAN, EC, (MDA), superoxide dismutase (SOD), nitric oxide (NO2- / NO3-), TXB2, 6-keto-prostaglandin F1α PGF1α) content and TXB2 / 6-keto-PGF1α ratio. Results: Compared with the model group, the contents of ATP, EC, NO2- / NO3- and 6-keto-PGF1α and the activities of SOD in liver tissue of LGT group, L-Arg group and LGT + L-Arg group were significantly increased And P <0.01). The contents of MDA and TXB2 and the ratio of TXB2 / 6-keto-PGF1α were significantly decreased (P <0.05 and P <0.01). Conclusions: LGT combined with L-Arg can improve the energy metabolism of liver in rats with ischemia-reperfusion injury by decreasing the levels of oxygen free radicals, increasing the level of nitric oxide and correcting the balance between TXA2 and PGI2.