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本文报道用光镜半定量和显微光度计定量分析研究了豚鼠胃肠壁内神经丛神经元的几种酶的组织化学反应。结果表明,神经元的碱性磷酸酶(AlP)、酸性磷酸酶(AcP)、5′-核苷酸酶(5′-Nase)、硫胺素焦磷酸酶(TPPase)、非特异性酯酶(NsE)和胆碱乙酰转移酶(ChAT)反应强弱明显不等。消化道不同节段或不同部位神经元的单胺氧化酶(MAO)、氨基肽酸(AP)和乙酰胆碱酯酶(AChE)反应虽有差别,但却显阳性反应,同一神经节内各神经元的反应比较近似。胃肠各段壁内神经丛中50～66%神经元呈ChAT强阳性反应,这些细胞可能为胆碱能神经元。整个消化道粘膜下丛与肠肌丛神经元相比,除NsE外,另几种酶均有高度显著差异。粘膜下丛神经元AcP和AP反应较强,肠肌丛神经元AlP、5′-Nase、TPPase、MAO、ChAT和AChE反应较强,胃壁内神经丛不如肠道的发达。尤其是胃粘膜下丛只见少数单个散在的神经元,它们的各种酶组织化学反应均较弱。各段肠中,以十二指肠和近端结肠壁内神经丛神经元的各种酶组织化学反应较强。上述结果表明,消化道不同部位以及同一部位不同类型的神经元在代谢和功能上有明显的差别。
In this paper, the histochemical reaction of several enzymes in the neuro-plexus neurons in the gastrointestinal wall of guinea pigs was quantitatively studied by light microscopy semi-quantitative and spectrophotometer. The results showed that neuronal AlP, AcP, 5’-Nase, TPPase, non-specific esterase NsE) and choline acetyltransferase (ChAT) reaction intensity was significantly different. MAO, AP and AChE responses of neurons in different segments or different parts of digestive tract were different, but they were positive, and the responses of neurons in the same ganglia were compared approximate. Gastric segments of the wall of the plexus in 50 to 66% neurons ChAT strong positive reaction, these cells may be cholinergic neurons. Compared with the myenteric plexus neurons in the entire gastrointestinal submucosal plexus, in addition to NsE, the other enzymes were highly significant difference. The responses of AcP and AP in submucosal plexus neurons were stronger. The reaction of AlP, 5’-Nase, TPPase, MAO, ChAT and AChE was stronger in the myenteric plexus neurons. In particular, gastric mucosal plexus only see a small number of single scattered neurons, their various enzymatic histochemical reactions are weak. Various sections of the intestine, to the duodenum and proximal colon wall plexus neurons of various enzyme tissue chemical reaction stronger. The above results show that different parts of the digestive tract and the same type of different types of neurons in the metabolism and function of significant differences.