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目的 :探讨DNA聚合酶Iota(Polι)与食管鳞癌淋巴结转移的关系。方法 :采用实时荧光定量PCR方法检测Polι基因在食管鳞癌组织中的表达,并使用Mann-Whitney U检验方法分析Polι与食管鳞癌淋巴结转移之间的关系;利用免疫组织化学染色法检测Polι及Nm23蛋白在食管鳞癌组织中的表达,并使用Spearman相关分析研究食管鳞癌中Polι与Nm23表达的相关性。向食管癌细胞TE-1和ECA-109转染Polι表达载体,上调细胞中Polι的表达,Realtime-PCR检测Polι和Nm23 m RNA的表达水平,并通过Transwell侵袭实验分析细胞的侵袭能力。结果:在食管鳞癌临床组织标本中,Polι表达与淋巴结转移相关(P<0.01),与Nm23蛋白表达呈负相关(R=-0.481,P<0.05)。上调Polι的表达增强了TE-1和ECA-109细胞的侵袭能力,差异有统计学意义(P均<0.05),同时抑制了细胞中Nm23 m RNA的表达,差异有统计学意义(P<0.001)。结论:Polι的表达与食管鳞癌的转移密切相关,其可能通过下调Nm23的表达来促进肿瘤转移。
Objective: To investigate the relationship between DNA polymerase Iota (Polι) and lymph node metastasis in esophageal squamous cell carcinoma. Methods: The expression of Pol1 gene in esophageal squamous cell carcinoma was detected by real-time fluorescence quantitative PCR. The relationship between Polι and lymph node metastasis of esophageal squamous cell carcinoma was analyzed by Mann-Whitney U test. Immunohistochemical staining was used to detect Polι and Nm23 protein expression in esophageal squamous cell carcinoma and Spearman correlation analysis of esophageal squamous cell carcinoma Polι and Nm23 expression. The Poli expression vector was transfected into esophageal cancer cells TE-1 and ECA-109 to up-regulate the expression of Poll in cells. Realtime-PCR was used to detect the expression of Poll and Nm23 mRNA. Transwell invasion assay was used to analyze the invasion ability of cells. Results: The expression of Polι in esophageal squamous cell carcinoma tissue was correlated with lymph node metastasis (P <0.01) and negatively with the expression of Nm23 protein (R = -0.481, P <0.05). The up-regulation of Poll expression enhanced the invasion ability of TE-1 and ECA-109 cells (all P <0.05), and inhibited the expression of Nm23 mRNA in the cells (P <0.001 ). Conclusion: The expression of Pol is closely related to the metastasis of esophageal squamous cell carcinoma, which may promote tumor metastasis by down-regulating the expression of Nm23.