论文部分内容阅读
目的研究婴幼儿感染的非伤寒沙门菌的分子流行病学特点和耐药情况。方法收集上海地区腹泻婴幼儿粪便标本,进行沙门菌的分离培养,生化鉴定,血清型分型,分子分型和药物敏感性检测实验。结果 583份婴幼儿粪便中分离出沙门菌共19株,主要为肠炎沙门菌和鼠伤寒沙门菌。肠炎沙门菌脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)带型相似度较高(88.7%),且主要带型为上海近年流行的优势带型。药物敏感性实验结果显示,菌株对测试的15种抗生素的总体耐药率为78.95%(15/19),对萘啶酸(68.42%)和氨苄西林(63.20%)的耐药率较高,对环丙沙星(10.53%)和庆大霉素(10.53%)的耐药率较低,对三代头孢类抗生素的耐药率较高(40%)。菌株耐药谱分布广泛,发现多重耐药菌株13株。结论本研究中,婴幼儿感染的非伤寒沙门菌耐药情况比较严重;不同血清型和分子分型的菌株耐药性差异无统计学意义。
Objective To study the molecular epidemiology and drug resistance of non-typhoid Salmonella in infants and young children. Methods Stool specimens of diarrhea infants and young children in Shanghai were collected for isolation and culture of Salmonella, biochemical identification, serotyping, molecular typing and drug susceptibility testing. Results A total of 19 strains of Salmonella were isolated from 583 infants excrement, mainly Salmonella enteritidis and Salmonella typhimurium. Salmonella enteritidis pulsed-field gel electrophoresis (PFGE) band pattern similarity is high (88.7%), and the main band type is the prevalence of belt in Shanghai in recent years. The drug susceptibility test results showed that the overall resistance rates of the strains to the 15 tested antibiotics were 78.95% (15/19) and the rates of resistance to nalidixic acid (68.42%) and ampicillin (63.20%) were high, The rate of resistance to ciprofloxacin (10.53%) and gentamicin (10.53%) was low, and the rate of resistance to third generation cephalosporins was high (40%). The strain of drug-resistant spectrum is widely distributed and found 13 strains of multi-drug resistant strains. Conclusion In this study, non-typhoid Salmonella infections in infants and young children were more serious. There was no significant difference in drug resistance between strains of different serotypes and molecular typing.