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目的:探讨SZJ对淀粉样β蛋白25-35(Aβ25-35)诱导的SH-SY5Y细胞凋亡的保护作用机制。方法:以Aβ25-35诱导SH-SY5Y细胞凋亡,采用MTT比色分析测定细胞存活率,应用Western blot检测Aβ25-35以及SZJ/APP17肽对SH-SY5Y细胞Bax、Bcl-2、Caspase-3表达的影响。结果:25μmol/L的Aβ25-35减低SH-SY5Y细胞存活率,SZJ/APP17肽预处理24h可提高SH-SY5Y细胞存活率,相同浓度Aβ25-35 SZJ预处理组与非处理组比较差异显著(P<0.05);25μmol/L Aβ25-35处理的SH-SY5Y细胞Bax表达增加,Bcl-2表达减少,Caspase-3的表达增高,SZJ/APP17肽预处理能抑制其表达升高,与非预处理组比较差异显著(P<0.05)。结论:SZJ可对抗Aβ25-35对SH-SY5Y细胞的毒性作用,其机制可能与抑制Bax、Caspase-3的表达,促进Bcl-2的表达有关。
Objective: To investigate the protective mechanism of SZJ on apoptosis of SH-SY5Y cells induced by amyloid β-protein 25-35 (Aβ25-35). Methods: Apoptosis of SH-SY5Y cells was induced by Aβ25-35. Cell viability was determined by MTT colorimetric assay. Western blot was used to detect Aβ25-35 and SZJ/APP17 peptides against SH-SY5Y cells Bax, Bcl-2, Caspase-3. The effect of expression. Results: 25μmol/L Aβ25-35 decreased the survival rate of SH-SY5Y cells. SZJ/APP17 peptide pretreatment for 24h could increase the survival rate of SH-SY5Y cells. The same concentration of Aβ25-35SZJ pretreatment group had significant difference compared with the non-treatment group. P<0.05); Bax expression in SH-SY5Y cells treated with 25μmol/L Aβ25-35 was increased, Bcl-2 expression was decreased, and Caspase-3 expression was increased. Pretreatment with SZJ/APP17 peptide inhibited the increased expression of Bax. The treatment group was significantly different (P<0.05). Conclusion: SZJ can counteract the toxic effects of Aβ25-35 on SH-SY5Y cells. The mechanism may be related to the inhibition of Bax, Caspase-3 expression and the promotion of Bcl-2 expression.