论文部分内容阅读
目的探讨滑膜肉瘤肿瘤细胞中基质金属蛋白酶-2(MMP2-)和金属蛋白酶组织抑制因子-2(TIMP-2)的表达情况及其预后意义。方法采用免疫组织化学SP法检测72例滑膜肉瘤肿瘤细胞中MMP-2、TIMP-2的表达情况,收集每例临床病理学参数并统计生存率、以CD31标记检测微血管密度(MVD)并分析与生存率的关系。结果(1)MMP-2、TIMP-2的表达阳性率分别为84.7%(61/72)和83.3%(60/72),二者表达强度呈负相关关系(r=-0.290,P=0.013)。(2)有转移病例出现MMP2高表达和TIMP2低表达的比例明显高于无转移病例(分别P=0.010,P=0.002)。(3)MMP2高表达病例的MVD明显大于其低表达者(P=0.005),TIMP2高表达病例的MVD明显小于其低表达者(P=0.048)。(4)单因素和多因素生存分析均显示TIMP2低表达与患者预后不良有关(分别P=0.002,P=0.016)。结论MMP-2和TIMP-2表达异常可能与滑膜肉瘤的转移及肿瘤血管生成有关,TIMP2低表达可能提示患者预后不良。
Objective To investigate the expression of matrix metalloproteinase-2 (MMP2-) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in synovial sarcoma tumor cells and their prognostic significance. Methods The expression of MMP-2 and TIMP-2 in 72 cases of synovial sarcoma was detected by immunohistochemical SP method. The clinicopathological parameters were collected and the survival rate was calculated. The MVD was detected by CD31 labeling Relationship with survival rate. Results (1) The positive rates of MMP-2 and TIMP-2 were 84.7% (61/72) and 83.3% (60/72), respectively. There was a negative correlation between the expression of MMP-2 and TIMP- ). (2) The proportion of high expression of MMP2 and low expression of TIMP2 in metastatic cases was significantly higher than that in non-metastatic cases (P = 0.010, P = 0.002, respectively). (3) The MVD of patients with high expression of MMP2 was significantly higher than that of patients with low expression (P = 0.005). The MVD of patients with high TIMP2 expression was significantly lower than those with low expression (P = 0.048). (4) Univariate and multivariate survival analyzes showed that the low expression of TIMP2 was associated with poor prognosis (P = 0.002, P = 0.016, respectively). Conclusion The abnormal expression of MMP-2 and TIMP-2 may be related to the metastasis of synovial sarcoma and tumor angiogenesis. The low expression of TIMP2 may indicate the poor prognosis of patients.