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目的了解四川地区2009年副溶血弧菌食物中毒疫情分离株和监测食品样品分离菌株菌型分布、致病力及分子分型特征,为四川地区副溶血性弧菌疾病防治提供科学依据。方法采用血清玻片凝集试验对2009年从食物中毒疫情和常规监测食品中分离的副溶血孤菌菌株进行血清分型、应用PCR方法进行耐热直接溶血素基因(tdh)和耐热直接溶血素相关基因(trh)的检测、小鼠腹腔注射进行致病力实验和进行脉冲场凝胶电泳(PFGE)分析等。结果 40株菌分属于5个血清群,疫情分离株分属于O3和O4群,监测分离株主要为O1群占43.75%(7/16),其中2起疫情分离到多个血清型的菌株;所有菌株trh基因均为阴性,其中20株携带tdh毒力基因,携带tdh毒力基因和不携带tdh、tlh毒力基因的菌株均有致病力。40株菌通过PFGE分型,共得到29个带型,同一疫情分离株具有多个PFGE型别。.结论四川省食品分离株和暴发疫情无太多联系,食品分离株血清型和PFGE型别呈多样性。对于从同一起暴发疫情中检出的不同血清型和不同PFGE型别的副溶血性弧菌需要确认各菌株与暴发的关系。
Objective To understand the distribution, pathogenicity and molecular typing of isolates of Vibrio parahaemolyticus in Sichuan Province in 2009 and to provide a scientific basis for the prevention and control of Vibrio parahaemolyticus in Sichuan Province. Methods Serum type of coagulase test was used to classify the strains of parahaemolyticus isolated from the food poisoning epidemic and routine monitoring foodstuffs in 2009. The thermostable direct hemolysin gene (tdh) and heat-resistant direct hemolysin Related genes (trh) detection, mouse intraperitoneal injection pathogenicity experiments and pulsed-field gel electrophoresis (PFGE) analysis. Results 40 isolates belonged to 5 serogroups. The isolates belonged to O3 and O4 isolates. The majority of isolates in the isolates belonged to 43.75% (7/16) O1 isolates, of which 2 serotypes were isolated from 2 outbreaks. All strains of trh gene were negative, of which 20 strains carrying tdh virulence genes, carrying tdh virulence genes and strains carrying no tdh, tlh virulence genes have pathogenicity. Forty strains were identified by PFGE, and a total of 29 bands were obtained. The same isolate had multiple PFGE types. .Conclusion There is not much connection between food isolates and outbreaks in Sichuan Province. The serotypes and PFGE types of food isolates are diversified. For strains of Vibrio parahaemolyticus of different serotypes and different PFGE types detected from the same outbreak, it is necessary to confirm the relationship between each strain and the outbreak.