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目的:为寻找抗日本血吸虫虫卵成熟疫苗候选分子提供实验依据。方法:用日本血吸虫未成熟卵可溶性抗原(SIEA)和成虫冷浸抗原经皮肤多次重复注射小鼠,再感染后49天收集小鼠肝内虫卵。聚乙二醇沉淀虫卵内抗原抗体复合物,应用EITB分析该抗原抗体复合物。结果:发现各组抗原抗体复合物的组份为67KD、54KD或40KD,其中54KD为诱导保护性免疫的各组样品共有组分。结论:推测54KD抗原分子在诱导宿主产生抗卵成熟的保护力可能起着重要作用
Objective: To provide experimental evidence for finding candidate antigens against Schistosoma japonicum egg mature vaccine. Methods: Mice were repeatedly injected with Schistosoma japonicum immature egg soluble antigen (SIEA) and adult cold-soaked antigen through the skin for several times, and the mice liver eggs were harvested 49 days after infection. Polyethylene glycol was used to precipitate the intra-egg antigen antibody complex, and the antigen-antibody complex was analyzed by EITB. Results: The components of the antigen-antibody complex of each group were found to be 67KD, 54KD or 40KD, of which 54KD was the common component of each sample that induced protective immunity. Conclusion: It is speculated that the 54KD antigen may play an important role in inducing the protective ability of the host against egg maturation