①目的探讨原位杂交检测人乳头瘤病毒（ＨＰＶ）对尖锐湿疣的病因诊断意义及原位杂交的方法学。②方法用地高辛标记原位杂交技术，对４５例临床疑为尖锐湿疣的组织进行了ＨＰＶ６Ｂ／１１ＤＮＡ检测。为减轻非特异性背景染色并获得最大杂交敏感性，对消化酶、变性及杂交温度和时间、洗涤液、显色剂等原位杂交方法进行了研究。③结果３２例病理诊断为尖锐湿疣者均呈阳性，４例病理疑诊为尖锐湿疣者呈阴性，９例病理诊断为假性湿疣者均为阴性。④结论ＨＰＶ原位杂交对尖锐湿疣的诊断更具可靠性 Objective To investigate the etiological diagnosis of condyloma acuminatum by in situ hybridization (HPV) detection and the methodology of in situ hybridization. Methods The digoxin-labeled in situ hybridization was used to detect the HPV6B / 11 DNA in 45 cases of clinically suspected condyloma acuminatum. In order to alleviate non-specific background staining and obtain the maximum hybridization sensitivity, in situ hybridization methods such as digestive enzymes, denaturation and hybridization temperature and time, washing liquid and chromogen were studied. Results 32 cases were diagnosed as condyloma acuminatum positive, 4 cases were diagnosed as condyloma acuminata were negative, 9 cases were pathologically diagnosed as false genital warts were negative. ④ Conclusion HPV in situ hybridization of genital warts diagnosis more reliable