论文部分内容阅读
目的:研究荭草苷对犬基底动脉舒张特征的影响及其机制。方法:制备狗基底动脉血管环,固定于盛有K-H液的恒温肌槽内,并观察等长时血管张力的变化。结果:荭草苷使KCl和无钙中CaCl2及NA的量效曲线明显右移,使肌条敏感性和最大收缩反应明显降低。Nω-L-硝基精氨酸、甲烯兰及去细胞内皮,可部分阻断荭草苷的舒张血管作用。反应兰-2和氯化四乙胺及吲哚美辛对荭草苷的舒张作用没有影响。结论:荭草苷时离体主动脉平滑肌的舒张作用与内皮有关,而与环氧化酶途径(前列腺素类物质)、嘌呤类受体和钾离子通道无关荭草苷抑制了血管平滑肌受体依赖性Ca2+通道及电压依赖性Ca2+通道,其抑制血管平滑肌依内钙与依外钙性收缩可能是荭草苷舒张血管平滑肌的主要机制。
Objective: To study the effect of orientin on diastolic characteristics of basilar artery in dogs and its mechanism. Methods: The basilar artery rings of dogs were prepared and fixed in the thermostatic muscle tank filled with K-H solution. The changes of vascular tension were observed at the same time. Results: Triterpene glycosides made CaCl 2 and calcium free CaCl 2 and NA in the right dose-effect curve shifted to the right, so that the sensitivity and maximum contraction of muscle strips significantly reduced. Nω-L-nitroarginine, methylene blue and acellular endothelium, can partially block the vasodilator effect of vinca glycosides. Reactive blue-2 and tetraethylammonium chloride and indomethacin did not affect the relaxation of oryzanol. CONCLUSION: The vasodilatation of ex vivo aorta smooth muscle during tardive glycosides is related to the endothelium, but not to the cyclooxygenase pathway (prostaglandins), purinergic receptors and potassium channels. Triflucone inhibits vascular smooth muscle receptors Dependent Ca2 + channels and voltage-dependent Ca2 + channels, which inhibit vascular smooth muscle intracellular calcium and extracellular calcium contraction may be the main mechanism of vinca glycosides relaxing vascular smooth muscle.