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分析了10个省市自治区的30个群体的青杨(Populus cathayana Rehd.),共169株,用RAPD方法分析群体间和群体内的DNA多态性。结果显示群体间的DNA多态度远高于群体内的,而且东部群体与西部群体间存在较大的遗传差异,相邻产地之间的遗传差异相对较小。聚类分析结果表明,东、西部群体已出现明显分化。整个聚类图与青杨的地理分布区大致相对应。西部群体内DNA多态性比东部群体高,因此西部群体在研究青杨的起源与演化中将显得更为重要。按照多样性中心与起源中心的关系,青杨可能起源于我国西北部,逐渐向东扩散,形成现代分布格局。
A total of 169 Populus cathayana Rehd. Populations from 30 populations in 10 provinces, municipalities and autonomous regions were analyzed. DNA polymorphism among populations and populations was analyzed by RAPD method. The results showed that the DNA polymorphism among the populations was much higher than that within the population, and there was a large genetic difference between the eastern population and the western population, and the genetic differences between the neighboring areas were relatively small. Cluster analysis showed that the eastern and western groups have been significantly differentiated. The entire cluster map roughly corresponds to the geographical distribution of the Poplar. Western populations have higher DNA polymorphism than the eastern population, so the western populations will become even more important in the study of the origin and evolution of the Poplar. According to the relationship between centers of diversity and centers of origin, Qingyang may have originated in northwestern China and gradually spread eastward to form a modern distribution pattern.