Ling和Gerard于1949年首先用玻璃微电极记录细胞内电位,并成功用于记录各种可兴奋组织包括心脏细胞,该法是在非生理条件下进行的,即组织离体、固定并用表面灌流在标本槽内进行记录。为了克服上述缺点,Woodbury等用浮置式玻璃微电极记录跳动组织的细胞内电位。此法曾被D.Durrer实验室用于记录在体和离体灌流猪心缺血时的心肌细胞电活.1979年Akiyama等用运动补偿电子电路,使电极固定装置随心脏跳动而自动上下,因此,常能在一个细胞内稳定记录高达10分钟,我们在家兔自发性呼吸条件下,将浮置式玻璃微电极加以改进,不难达到稳定记录15分钟。但其他常用实验动物,如犬、猫,其两侧胸膜腔的 Ling and Gerard first recorded intracellular potentials with glass microelectrodes in 1949 and were successfully used to record a variety of excitable tissues, including cardiac cells, under non-physiological conditions, ie, the tissue was isolated, fixed and surface-perfused Record in the specimen tank. To overcome these shortcomings, Woodbury et al. Used floating glass microelectrodes to record the intracellular potential of bounced tissue. This method was D. Durrer laboratory was used to record in vitro and in vitro perfused pig heart ischemia myocardial cell activity in 1979 Akiyama and other motion compensation with electronic circuits so that the electrode fixture with the heart beats automatically up and down, As a result, stable recordings of up to 10 minutes can be performed in a single cell. We improved the floating glass microelectrode under spontaneous breathing in rabbits and achieved a stable record of 15 minutes. But other commonly used laboratory animals, such as dogs, cats, on both sides of the pleural cavity