目的:制备并鉴定白喉毒素单克隆抗体杂交瘤细胞株,为白喉的快速检测及致病机制的研究提供有力的抗体工具。方法:用白喉类毒素做抗原免疫BALB/c小鼠,取其脾细胞与小鼠骨髓瘤细胞融合,获得分泌高滴度针对白喉类毒素的杂交瘤细胞株,测定单抗免疫球蛋白亚类及单抗效价,用间接ELISA和Western-blot检测单克隆细胞株的特异性。结果:通过细胞融合和克隆化,筛选出3株持续分泌抗白喉类毒素单克隆抗体的杂交瘤细胞株1E12、1E11、1G10。间接ELISA和Western-blot检测结果表明1E12、1E11、1G10可以和白喉类毒素发生特异性反应。结论:成功制备了抗白喉类毒素单克隆抗体,为制备免疫诊断试剂盒和抗体药物的开发奠定了基础。 OBJECTIVE: To prepare and identify the diphtheria toxin monoclonal antibody hybridoma cell line for the rapid detection and pathogenesis of diphtheria to provide a powerful antibody tool. Methods: BALB / c mice were immunized with diphtheria toxoid antigen and their spleen cells were fused with mouse myeloma cells. The hybridoma cell lines secreting high titer of diphtheria toxoid were obtained and immunoglobulin subclasses And monoclonal antibody titer, using indirect ELISA and Western-blot detection of monoclonal cell line specificity. Results: Three hybridoma cell lines, 1E12, 1E11, and 1G10, secreting monoclonal antibodies against diphtheria toxoid were selected by cell fusion and cloning. Indirect ELISA and Western-blot results showed that 1E12, 1E11 and 1G10 could specifically react with diphtheria toxoid. Conclusion: The anti-diphtheria toxoid monoclonal antibody was successfully prepared, which laid the foundation for the preparation of immunodiagnostic kits and the development of antibody drugs.