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生活在宿主植物里的内生真菌是很重要的药用资源。喜树是中国的传统药用植物。从喜树植物中分离得到了大约50种菌株,其中一株产喜树碱的菌株通过形态学鉴定为青霉属,这是首次在喜树植物中发现产喜树碱的青霉属菌株。为研究简单序列重复相关序列扩增多态性(SRAP)方法在喜树内生真菌中应用的可行性,选择了十株喜树内生真菌进行SRAP多态性分析。SRAP引物共扩增出1 295条带,而这些菌株也被分为三大类。这些结果表明,SRAP研究喜树内生真菌具有高效性,是讨论喜树内生真菌的遗传多样性的有效方法。
Endophytic fungi living in host plants are important medicinal resources. Camptotheca acuminata is a traditional Chinese medicinal plant. About 50 strains were isolated from Camptotheca acuminatum. One camptothecin-producing strain was morphologically identified as Penicillium. This is the first Penicillium strain to be found in camptotheca acuminata. In order to study the feasibility of SRAP in the application of endophytic fungi of Camptotheca acuminata, 10 endophytic Camptotheca acuminatum strains were selected for SRAP analysis. A total of 1 295 bands were amplified by SRAP primers, and these strains were also divided into three broad categories. These results indicate that the high efficiency of SRAP in studying endophytic fungi of Camptotheca acuminata is an effective method to discuss the genetic diversity of endophytic fungi in Camptotheca acuminatum.