目的:建立复方维生素B_6凝胶剂中维生素B_6与醋酸氟轻松的含量测定方法。方法:采用HPLC法,色谱柱:Lichrospher CN C_(18)柱(250 mm×4.6 mm,5μm);流动相:庚烷磺酸钠溶液(称取6.8 g磷酸二氢钾和1.0 g庚烷磺酸钠,加水溶解并稀释至1 000 ml)-甲醇-三乙胺(35∶65∶0.2),用磷酸调pH至3.2;检测波长:238 nm;柱温:25℃;进样量:20μl。结果:维生素B_6在15.0~300.0μg·ml-1浓度范围内线性关系良好(r=1.0000),平均回收率为99.65%,RSD为0.3%(n=9);醋酸氟轻松在0.4~8.0μg·ml-1浓度范围内线性关系良好(r=0.999 0),平均回收率为99.35%,RSD为0.85%(n=9)。结论:该方法简便,重复性好,测定的结果准确,可用于复方维生素B6凝胶剂的含量测定。 Objective: To establish a method for the determination of vitamin B 6 and fluocinonide in compound vitamin B 6 gel. Methods: The HPLC method was adopted. The column was Lichrospher CN C 18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was heptane sodium sulfonate (6.8 g potassium dihydrogen phosphate and 1.0 g heptane sulfonate Sodium acetate, dissolved in water and diluted to 1 000 ml) -methanol-triethylamine (35:65:0.2) adjusted to pH 3.2 with phosphoric acid; detection wavelength: 238 nm; column temperature: 25 ° C .; injection volume: . Results: The calibration curve of vitamin B 6 was linear in the concentration range of 15.0-300.0 μg · ml -1 (r = 1.0000), with an average recovery of 99.65% and a RSD of 0.3% (n = 9) · The linear relationship was good (r = 0.999 0) with an average recovery of 99.35% and a RSD of 0.85% (n = 9) in the concentration range of ml-1. Conclusion: The method is simple, reproducible, and accurate. It can be used to determine the content of compound vitamin B6 gel.