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建立了测定人血浆中芦氟沙星质量浓度的高效液相色谱法 ,血浆用二氯甲烷提取 3次 ,以 UltrasphereODS(4.6mm i.d.× 2 5 0 mm)为色谱柱 ,流动相为甲醇 -1 0 mmol/L 溴化四丁铵 -三乙胺 (体积比为 3 2∶ 68∶0 .5 ) ,用磷酸调 p H2 .8,检测波长 2 95 nm,流速为 1 .2 m L/min,以培氟沙星为内标。血浆中芦氟沙星的线性范围为 0 .1～ 1 0 mg/L ,最低检测质量浓度为 0 .0 5 mg/L ,回收率为 99.7% ,日内、日间 RSD分别为 2 .3 3 %和3 .83 %。方法简便、快速、准确 ,适用于人血浆中芦氟沙星质量浓度的测定。
A high performance liquid chromatographic method was established for the determination of the concentration of rufloxacin in human plasma. The plasma was extracted three times with dichloromethane. The column was Ultrasphere ODS (4.6 mm id × 250 mm) and the mobile phase was methanol-1 0 mmol / L tetrabutylammonium bromide-triethylamine (3: 68: 0.5 by volume), adjusted to pH 2.2 with phosphoric acid, detected at a wavelength of 955 nm at a flow rate of 1.2 m L / min Pefloxacin as an internal standard. The linear range of rufloxacin in plasma ranged from 0.1-1.0 mg / L, the lowest detection concentration was 0.05 mg / L and the recovery rate was 99.7%. The intra-day and inter-day RSD were 2.333 % And 3.83%. The method is simple, rapid and accurate and is suitable for the determination of rufloxacin in human plasma.