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Objective To introduce a rapid simple staining method for sperm morphology.Methods Liquifiedsemen was on the glass, fixed by methanol, treated with phosphonic acid buffer, stained by A, B staining solution separately, and rinsed by running water. Results After staining, the whole sper-matozoid was very clear. The cephalic zone of spermatozoid head , acrosome area , was colored pink; its caudal zone colored violet red or violet blue. The body and tail of spermatozoid colored pink or light blue. Normal and abnormal morphology of spermatozoid could be differentiated easily. The sloughed spermatogenic cells and white blood cells could be differentiated also. Conclusion This rapid staining method of semen smear can produce the same effect of other usual staining method , but the staining time is shorter and the procedure is simpler.
Objective To introduce a rapid simple staining method for sperm morphology. Methods Liquifiedsemen was on the glass, fixed by methanol, treated with phosphonic acid buffer, stained by A, B staining solution separately, and rinsed by running water. Results After staining, the whole sper-matozoid was very clear. The cephalic zone of spermatozoid head, acrosome area, was colored pink; its caudal zone colored violet red or violet blue. The body and tail of spermatozoid colored pink or light blue. be differentiated easily. The sloughed spermatogenic cells and white blood cells could be differentiated also.. Conclusion This rapid staining method of semen smear can produce the same effect of other usual staining method, but the staining time is shorter and the procedure is simpler.