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目的:探讨需钙蛋白酶抑制剂I(CI-I)对LPS攻击RAW264.7细胞后iκBα表达和细胞因子分泌的影响。方法:用CI-I预处理RAW264.7细胞1h后,再用LPS攻击,分别用Westernblot和ELISA检测RAW264.7细胞iκBα蛋白表达和TNF-α、IL-6的分泌。结果:CI-I预处理RAW264.7细胞后,可抑制LPS导致的iκBα表达降低。LPS攻击RAW264.7细胞后4h和8h,TNF-α和IL-6的分泌均增加,CI-I和地塞米松(DEX)可抑制该效应,并具有协同抑制作用。结论:CI-I和DEX可抑制LPS攻击后RAW264.7细胞中iκBα表达和炎性细胞因子分泌,从而有助于减轻细胞损伤。
Objective: To investigate the effect of calpain inhibitor I (CI-I) on iκBα expression and cytokine secretion after LPS challenge in RAW264.7 cells. Methods: RAW264.7 cells were pretreated with CI-I for 1 h and then challenged with LPS. Western blot and ELISA were used to detect the expression of iκBα and the secretion of TNF-α and IL-6 in RAW264.7 cells. Results: CI-I pretreatment RAW264.7 cells, can inhibit LPS induced iκBα expression decreased. After LPS challenge RAW264.7 cells 4h and 8h, the secretion of TNF-α and IL-6 increased, CI-I and dexamethasone (DEX) can inhibit this effect, and with synergistic inhibition. Conclusions: CI-I and DEX can inhibit the expression of iκBα and the secretion of inflammatory cytokines in RAW264.7 cells after LPS challenge, which may help to reduce cell injury.