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在已知的共刺激分子中,B7分子(包括B7-1和B7-2分子)与它的反向受体CD28的相互作用被认为对免疫应答是十分重要的,同时,亦已显示B7-CD28的相互作用对CD8~+CTL的产生是必要的.为了调查B7分子的表达是否可以使人多发性骨髓瘤细胞诱导出CD8~+的CTL依赖的抗肿瘤反应,我们构建了含B7-1基因的逆转录病毒载体PTG5192,通过Lipofectin法将含有B7-1基因的PTG5192导入包装细胞293E中,用G418进行选择,经流式细胞仪筛选得到高表达B7-1分子的293E细胞,保留其培养上清,然后用此培养上清
In known co-stimulatory molecules, the interaction of the B7 molecule (including the B7-1 and B7-2 molecules) with its counter-receptor CD28 is considered to be very important for the immune response, and B7- The interaction of CD28 is necessary for the production of CD8~+CTL. To investigate whether the expression of B7 molecules can induce CD8~+ CTL-dependent antitumor responses in multiple myeloma cells, we constructed B7-1 The retroviral vector PTG5192 of the gene was introduced into 293E packaging cells by the Lipofectin method and inserted into 293E packaging cells, selected by G418, and 293E cells highly expressing B7-1 molecule were screened by flow cytometry and cultured. The supernatant, then use this to cultivate the supernatant