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目的观察应用聚维酮碘软膏在大鼠绿脓杆菌感染创面生物被膜形成及细菌数的变化,从而探讨新型碘制剂对创面愈合的影响。方法14只大鼠创面模型接种带有GFP的野生型绿脓杆菌5×107cfu/创面,分成两组,治疗组术后2,4天分别用聚维酮碘软膏及单纯载体软膏(不含聚维酮碘)外敷,分别于术后2天,4天,6天用棉棒取渗出液,细菌计数,于术后6天处死大鼠,取组织观察组织内细菌数及生物被膜形成情况。结果聚维酮碘软膏治疗组渗出液及渗出液中的细菌数较对照组少,有差异显著性(P<0.05)且生物被膜较对照组生成少。结论聚维酮碘可减少溃疡创面细菌数,且减少生物被膜的形成,促进创面上皮化。
Objective To observe the formation of biofilm and the number of bacteria in the wound of Pseudomonas aeruginosa infected with Povidone iodine ointment, so as to explore the effect of new iodine preparations on wound healing. Methods Fourteen rat wound models were inoculated with 5 × 107 cfu / wound of wild-type Pseudomonas aeruginosa with GFP and divided into two groups. Povidone iodine ointment and simple carrier ointment (without poly Vital ketone iodine) topical, respectively, 2 days after operation, 4 days, 6 days with a cotton swab to take the exudate, bacteria count, 6 days after the operation of rats were killed, the number of tissues and biofilm formation was observed tissue . Results Povidone iodine ointment treatment group exudate and exudate bacteria than the control group, there was a significant difference (P <0.05) and biofilm formation less than the control group. Conclusion Povidone iodine can reduce the bacterial count of ulcer wounds and reduce the formation of biofilm and promote wound epithelization.