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基于细胞球的体外组织模型在生理病理机理和药物筛选方面具有重要的研究意义和广阔的应用前景,利用微纳生物制造技术可控构建细胞球已吸引了越来越多研究者的关注。微孔板法作为一种简单易行的细胞球构建方法经常被研究者采用,但目前的微孔板法存在结构制备和可控性不足、细胞种植不均匀以及细胞容易流失等问题。本文利用自行搭建的细胞打印技术制备载细胞的明胶微凝胶作为模板,构建了带有凹面微孔阵列的聚乙二醇二甲基丙烯酸酯水凝胶微孔板芯片并原位形成了乳腺癌细胞球。本方法操作灵活、可控性好,避免了细胞种植不均匀和细胞流失等问题,在组织工程、再生医学以及药物筛选等研究具有重要的应用价值。
Cell-based in vitro tissue models have important research significance and broad application prospects in the aspects of physiological and pathological mechanisms and drug screening. The controllable construction of cell spheres using micro / nano biological manufacturing technology has attracted more and more researchers’ attention. Microplate method is often used by researchers as a simple and easy method of constructing cell spheres. However, the current microplate method has some problems such as poor preparation and controllability, uneven cell growth and easy cell loss. In this paper, a self-built cell printing technique was used to prepare cell-loaded gelatin microgel as a template, and a polyethylene glycol dimethacrylate hydrogel microplate with a concave micropore array was constructed and formed in situ Cancer cell ball. The method has the advantages of flexible operation, good controllability, avoided problems of cell planting unevenness and cell loss, and has important application value in tissue engineering, regenerative medicine and drug screening.